Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 박은진 | * |
dc.date.accessioned | 2016-08-28T12:08:05Z | - |
dc.date.available | 2016-08-28T12:08:05Z | - |
dc.date.issued | 2008 | * |
dc.identifier.issn | 0882-2786 | * |
dc.identifier.other | OAK-5155 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/220079 | - |
dc.description.abstract | Purpose: The purpose of this study was to modify the method of platelet-rich plasma (PRP) preparation for obtaining optimal angiogenic potential and accelerate bone healing. Also, the potential synergistic effect of a suboptimal concentration of bone morphogenic protein-2 (BMP-2) and modified PRP (mPRP) on bone healing was evaluated in vivo. Materials and Methods: The angiogenic factor-enriched PRP, which included peripheral blood mononuclear cells (mostly lymphocytes and monocytes, excluding polymorphonuclear leukocytes [PMNs], was achieved by lowering concentrations of thrombin and CaCl 2, after pre-activation with shear stress using a table-top vortex machine and collagen. In vitro, endothelial cell migration activity in the mPRP group was compared to conventional PRP preparation using a modified Boyden chamber assay. In an animal study, PGA scaffold, PGA scaffold + mPRP, PGA scaffold + mPRP + rhBMP-2, and PGA scaffold + rhBMP-2 were applied to critical-sized calvarial defects in 28 nude rats. At 2 weeks, periosteal blood flow was measured using laser Doppler perfusion imaging, and bone formation was evaluated at 8 weeks by histology, dual energy x-ray absorptiometry, and micro-computed tomography. Results: mPRP induced faster migration of cord blood-derived outgrowth endothelial-like cells. In vivo, the group with mPRP with a low dose of rhBMP-2 showed significantly increased numbers of blood vessels at 2 weeks and notable synergistic effect on bone healing at 8 weeks as evaluated with histology, bone mineral density and bone mineral content, and pCT. Conclusion: The mPRP used in this study improved vascular perfusion around the defect and resulted in enhanced bone healing. Also, combining mPRP with a suboptimal dosage of rhBMP-2 improved bone formation and enhanced bone density. | * |
dc.language | English | * |
dc.title | Improved bone healing by angiogenic factor-enriched platelet-rich plasma and its synergistic enhancement by bone morphogenetic protein-2 | * |
dc.type | Article | * |
dc.relation.issue | 5 | * |
dc.relation.volume | 23 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 818 | * |
dc.relation.lastpage | 826 | * |
dc.relation.journaltitle | International Journal of Oral and Maxillofacial Implants | * |
dc.identifier.wosid | WOS:000260332100003 | * |
dc.identifier.scopusid | 2-s2.0-55349144153 | * |
dc.author.google | Park E.-J. | * |
dc.author.google | Kim E.-S. | * |
dc.author.google | Weber H.-P. | * |
dc.author.google | Wright R.F. | * |
dc.author.google | Mooney D.J. | * |
dc.contributor.scopusid | 박은진(22980964700) | * |
dc.date.modifydate | 20240301081003 | * |