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Inhibition of LPS-induced iNOS, COX-2 and cytokines expression by poncirin through the NF-κB inactivation in RAW 264.7 macrophage cells

Title
Inhibition of LPS-induced iNOS, COX-2 and cytokines expression by poncirin through the NF-κB inactivation in RAW 264.7 macrophage cells
Authors
Kim J.-B.Han A.-R.Park E.-Y.Kim J.-Y.Cho W.Lee J.Seo E.-K.Lee K.-T.
Ewha Authors
서은경한아름
SCOPUS Author ID
서은경scopus; 한아름scopusscopus
Issue Date
2007
Journal Title
Biological and Pharmaceutical Bulletin
ISSN
0918-6158JCR Link
Citation
vol. 30, no. 12, pp. 2345 - 2351
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
We previously reported that poncirin, a flavanone glycoside isolated from the EtOAc extract of the dried immature fruits of Poncirus trifoliata, is an anti-inflammatory compound that inhibits PGE 2 and IL-6 production. The present work was undertaken to investigate the molecular actions of poncirin in RAW 264.7 macrophage cell line. Poncirin reduced lipopolysaccharide (LPS)-induced protein levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) and the mRNA expressions of iNOS, COX-2, tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in a concentration- dependent manner, as determined by Western blotting and RT-PCR, respectively. Furthermore, poncirin inhibited the LPS-induced DNA binding activity of nuclear factor-κB (NF-κB). Moreover, this effect was accompanied by a parallel reduction in IκB-α degradation and phosphorylation that in by nuclear translocations of p50 and p65 NF-βB subunits. Taken together, our data indicate that anti-inflammatory properties of poncirin might be the result from the inhibition iNOS, COX-2, TNF-α and IL-6 expression via the down-regulation of NF-κB binding activity. © 2007 Pharmaceutical Society of Japan.
DOI
10.1248/bpb.30.2345
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약학대학 > 약학과 > Journal papers
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