Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 서석효 | - |
dc.contributor.author | 최신규 | - |
dc.date.accessioned | 2016-08-28T12:08:53Z | - |
dc.date.available | 2016-08-28T12:08:53Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 0014-2999 | - |
dc.identifier.other | OAK-4664 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/219968 | - |
dc.description.abstract | The effect of the selective inhibitor of Na+/Ca2+ exchanger (NCX), KB-R7943, on large-conductance Ca2+-activated K+ (BKCa) channels was examined in cultured human umbilical vein endothelial cells (HUVECs) and freshly isolated mouse aortic smooth muscle cells (MASMCs). In voltage-clamped cells, KB-R7943 reversibly activated BKCa currents in HUVECs and MASMCs. The EC50 of KB-R7943 for BKCa current activation in HUVECs was determined to be 6.78 ± 0.7 μM. In inside-out and outside-out patches, KB-R7943 markedly increased BKCa channel activity and slightly decreased single channel current amplitudes. In inside-out patches, KB-R7943 shifted the relationship between [Ca2+]i and open probability (Po) to the left; the [Ca2+]i required to evoke half-maximal activation changed from 1220 ± 68 nM (in the absence of KB-R7943) to 620 ± 199 nM (in the presence of 10 μM KB-R7943). In addition, KB-R7943 shifted the relationship between membrane potential and Po to the left; the membrane potential to evoke half-maximal activation changed from 76.86 ± 1.09 mV (in the absence of KB-R7943) to 49.62 ± 2.55 mV (in the presence of 10 μM KB-R7943). In conclusion, KB-R7943 was found to act as a potent BKCa channel activator, which increases the sensitivity of BKCa channels to cytosolic free Ca2+ and membrane potential, and thereby BKCa channel activity. These results should be considered when KB-R7943 is used as NCX blocker. © 2008 Elsevier B.V. All rights reserved. | - |
dc.language | English | - |
dc.title | The Na+/Ca2+ exchanger inhibitor KB-R7943 activates large-conductance Ca2+-activated K+ channels in endothelial and vascular smooth muscle cells | - |
dc.type | Article | - |
dc.relation.issue | 41277 | - |
dc.relation.volume | 582 | - |
dc.relation.index | SCI | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 35 | - |
dc.relation.lastpage | 41 | - |
dc.relation.journaltitle | European Journal of Pharmacology | - |
dc.identifier.doi | 10.1016/j.ejphar.2007.12.021 | - |
dc.identifier.wosid | WOS:000253877700005 | - |
dc.identifier.scopusid | 2-s2.0-39149125440 | - |
dc.author.google | Liang G.H. | - |
dc.author.google | Kim J.A. | - |
dc.author.google | Seol G.H. | - |
dc.author.google | Choi S. | - |
dc.author.google | Suh S.H. | - |
dc.contributor.scopusid | 서석효(55666113100) | - |
dc.contributor.scopusid | 최신규(12783276200) | - |
dc.date.modifydate | 20230901081001 | - |