Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 이서구 | * |
dc.contributor.author | 배윤수 | * |
dc.contributor.author | 이인혜 | * |
dc.date.accessioned | 2016-08-28T12:08:53Z | - |
dc.date.available | 2016-08-28T12:08:53Z | - |
dc.date.issued | 2008 | * |
dc.identifier.issn | 0021-9258 | * |
dc.identifier.other | OAK-4653 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/219966 | - |
dc.description.abstract | We have previously reported that central repeated units (CRUs) of Ahnak act as a scaffolding protein networking phospholipase Cγ and protein kinase C (PKC). Here, we demonstrate that an Ahnak derivative consisting of four central repeated units binds and activates PKC-α in a phosphatidylserine/1,2- dioleoyl-sn-glycerol-independent manner. Moreover, NIH3T3 cells expressing the 4 CRUs of Ahnak showed enhanced c-Raf, MEK, and Erk phosphorylation in response to phorbol 12-myristate 13-acetate (PMA) compared with parental cells. To evaluate the effect of loss-of-function of Ahnak in cell signaling, we investigated PKC activation and Raf phosphorylation in embryonic fibroblast cells (MEFs) of the Ahnak knock-out (Ahnak-/-) mouse. Membrane translocation of PKC-α and phosphorylation of Raf in response to PMA or platelet-derived growth factor were decreased in Ahnak null MEF cells compared with wild type MEFs. Several lines of evidence suggest that PKC-α activity is regulated through association with protein phosphatase 2A (PP2A). A co-immunoprecipitation assay indicated that the association of PKC-α with PP2A was disrupted in NIH3T3 cells expressing 4 CRUs of Ahnak in response to PMA. Consistently, Ahnak null MEF cells stimulated by PMA showed enhanced PKC-PP2A complex formation, and add-back expression of Ahnak into Ahnak null MEF cells abolished the PKC-PP2A complex formation in response to PMA. These data indicate that Ahnak potentiates PKC activation through inhibiting the interaction of PKC with PP2A. © 2008 by The American Society for Biochemistry and Molecular Biology, Inc. | * |
dc.language | English | * |
dc.title | Ahnak protein activates protein kinase C (PKC) through dissociation of the PKC-protein phosphatase 2A complex | * |
dc.type | Article | * |
dc.relation.issue | 10 | * |
dc.relation.volume | 283 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 6312 | * |
dc.relation.lastpage | 6320 | * |
dc.relation.journaltitle | Journal of Biological Chemistry | * |
dc.identifier.doi | 10.1074/jbc.M706878200 | * |
dc.identifier.wosid | WOS:000253779500038 | * |
dc.identifier.scopusid | 2-s2.0-44449175698 | * |
dc.author.google | In H.L. | * |
dc.author.google | Hee J.L. | * |
dc.author.google | Yoon S. | * |
dc.author.google | Je K.S. | * |
dc.author.google | Duk S.B. | * |
dc.author.google | Sue G.R. | * |
dc.author.google | Yun S.B. | * |
dc.contributor.scopusid | 이서구(7401852092) | * |
dc.contributor.scopusid | 배윤수(15031067200) | * |
dc.contributor.scopusid | 이인혜(26531358400) | * |
dc.date.modifydate | 20240423081003 | * |