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Extracellular NAD is a regulator for FcγR-mediated phagocytosis in murine macrophages

Title
Extracellular NAD is a regulator for FcγR-mediated phagocytosis in murine macrophages
Authors
Song E.-K.Lee Y.-R.Yu H.-N.Kim U.-H.Rah S.-Y.Park K.-H.Shim I.-K.Lee S.-J.Park Y.-M.Chung W.-G.Kim J.-S.Han M.-K.
Ewha Authors
이승진심인경
SCOPUS Author ID
이승진scopus
Issue Date
2008
Journal Title
Biochemical and Biophysical Research Communications
ISSN
0006-291XJCR Link
Citation
vol. 367, no. 1, pp. 156 - 161
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
NAD is available in the extracellular environment and elicits immune modulation such as T cell apoptosis by being used as the substrate of cell surface ADP-ribosyl transferase. However, it is unclear whether extracellular NAD affects function of macrophages expressing cell surface ADP-ribosyl transferase. Here we show that extracellular NAD enhances Fcγ receptor (FcγR)-mediated phagocytosis in J774A.1 macrophages via the conversion into cyclic ADP-ribose (cADPR), a potent calcium mobilizer, by CD38, an ADP-ribosyl cyclase. Extracellular NAD increased the phagocytosis of IgG-coated sheep red blood cells (IgG-SRBC) in J774A.1 macrophages, which was completely abolished by pretreatment of 8-bromo-cADPR, an antagonist of cADPR, or CD38 knockdown. Extracellular NAD increased basal intracellular Ca2+ concentration, which also was abolished by pretreatment of 8-bromo-cADPR or CD38 knockdown. Moreover, the chelation of intracellular calcium abolished NAD-induced enhancement of phagocytosis of IgG-SRBC. Our results suggest that extracellular NAD act as a regulator for FcγR-mediated phagocytosis in macrophages. © 2007 Elsevier Inc. All rights reserved.
DOI
10.1016/j.bbrc.2007.12.131
Appears in Collections:
약학대학 > 약학과 > Journal papers
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