Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 윤영대 | - |
dc.contributor.author | 박인영 | - |
dc.date.accessioned | 2016-08-28T12:08:43Z | - |
dc.date.available | 2016-08-28T12:08:43Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 0006-4971 | - |
dc.identifier.other | OAK-4074 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/219866 | - |
dc.description.abstract | Lck-interacting adaptor protein/Rlk/Itk-binding protein (Lad/RIBP) was previously identified as an adaptor protein involved in TCR-mediated T-cell activation. To elucidate the functions of Lad further, we here performed yeast 2-hybrid screening using Lad as bait and discovered that the G protein β subunit (Gβ) is a Lad-binding partner. Since the most well-known G protein-coupled receptor in T cells is the chemokine receptor, we investigated whether Lad is involved in chemokine signaling.We found that, upon chemokine treatment, Lad associated with Gβ in Jurkat T cells. Furthermore, ectopic expression of dominant-negative Lad or the reduction of endogenous Lad expression by siRNA impaired the chemokine-induced migration of T cells, indicating that Lad is required for chemokine-induced T-cell migration. Subsequent investigation of the signaling pathways revealed that, in response to chemokine, Lad associated with the tyrosine kinases Lck and Zap-70 and that Lad was essential for the activation of Zap-70. Moreover, Lad was required for the chemokine-dependent tyrosine phosphorylation of focal adhesion molecules that included Pyk2 and paxillin.Taken together, these data show that, upon chemokine stimulation, Lad acts as an adaptor protein that links the G protein β subunit to the tyrosine kinases Lck and Zap-70, thereby mediating T-cell migration. © 2007 by The American Society of Hematology. | - |
dc.language | English | - |
dc.title | The adaptor protein Lad associates with the G protein β subunit and mediates chemokine-dependent T-cell migration | - |
dc.type | Article | - |
dc.relation.issue | 12 | - |
dc.relation.volume | 109 | - |
dc.relation.index | SCI | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 5122 | - |
dc.relation.lastpage | 5128 | - |
dc.relation.journaltitle | Blood | - |
dc.identifier.doi | 10.1182/blood-2005-10-061838 | - |
dc.identifier.wosid | WOS:000247360200018 | - |
dc.identifier.scopusid | 2-s2.0-34249982918 | - |
dc.author.google | Park D. | - |
dc.author.google | Park I. | - |
dc.author.google | Lee D. | - |
dc.author.google | Young B.C. | - |
dc.author.google | Lee H. | - |
dc.author.google | Yun Y. | - |
dc.contributor.scopusid | 윤영대(7201731033) | - |
dc.contributor.scopusid | 박인영(7403243197;56302476200) | - |
dc.date.modifydate | 20230118102324 | - |