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dc.contributor.author윤영대-
dc.contributor.author박인영-
dc.date.accessioned2016-08-28T12:08:43Z-
dc.date.available2016-08-28T12:08:43Z-
dc.date.issued2007-
dc.identifier.issn0006-4971-
dc.identifier.otherOAK-4074-
dc.identifier.urihttp://dspace.ewha.ac.kr/handle/2015.oak/219866-
dc.description.abstractLck-interacting adaptor protein/Rlk/Itk-binding protein (Lad/RIBP) was previously identified as an adaptor protein involved in TCR-mediated T-cell activation. To elucidate the functions of Lad further, we here performed yeast 2-hybrid screening using Lad as bait and discovered that the G protein β subunit (Gβ) is a Lad-binding partner. Since the most well-known G protein-coupled receptor in T cells is the chemokine receptor, we investigated whether Lad is involved in chemokine signaling.We found that, upon chemokine treatment, Lad associated with Gβ in Jurkat T cells. Furthermore, ectopic expression of dominant-negative Lad or the reduction of endogenous Lad expression by siRNA impaired the chemokine-induced migration of T cells, indicating that Lad is required for chemokine-induced T-cell migration. Subsequent investigation of the signaling pathways revealed that, in response to chemokine, Lad associated with the tyrosine kinases Lck and Zap-70 and that Lad was essential for the activation of Zap-70. Moreover, Lad was required for the chemokine-dependent tyrosine phosphorylation of focal adhesion molecules that included Pyk2 and paxillin.Taken together, these data show that, upon chemokine stimulation, Lad acts as an adaptor protein that links the G protein β subunit to the tyrosine kinases Lck and Zap-70, thereby mediating T-cell migration. © 2007 by The American Society of Hematology.-
dc.languageEnglish-
dc.titleThe adaptor protein Lad associates with the G protein β subunit and mediates chemokine-dependent T-cell migration-
dc.typeArticle-
dc.relation.issue12-
dc.relation.volume109-
dc.relation.indexSCIE-
dc.relation.indexSCOPUS-
dc.relation.startpage5122-
dc.relation.lastpage5128-
dc.relation.journaltitleBlood-
dc.identifier.doi10.1182/blood-2005-10-061838-
dc.identifier.wosidWOS:000247360200018-
dc.identifier.scopusid2-s2.0-34249982918-
dc.author.googlePark D.-
dc.author.googlePark I.-
dc.author.googleLee D.-
dc.author.googleYoung B.C.-
dc.author.googleLee H.-
dc.author.googleYun Y.-
dc.contributor.scopusid윤영대(7201731033)-
dc.contributor.scopusid박인영(7403243197)-
dc.date.modifydate20200901081003-
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자연과학대학 > 생명과학전공 > Journal papers
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