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Establishment of an assay for P2X7 receptor-mediated cell death

Title
Establishment of an assay for P2X7 receptor-mediated cell death
Authors
Lee S.-Y.Jo S.Lee G.E.Jeong L.S.Kim Y.-C.Park C.-S.
Ewha Authors
정낙신
Issue Date
2006
Journal Title
Molecules and Cells
ISSN
1016-8478JCR Link
Citation
vol. 22, no. 2, pp. 198 - 202
Indexed
SCI; SCIE; SCOPUS; KCI WOS scopus
Abstract
The P2X7 receptor, an ATP-gated cation channel, induces cell death in immune cells and is involved in neurodegenerative diseases. Although the receptor plays various roles in these diseases, the cellular mechanisms involved are poorly understood and antagonists are limited. Here, the development of a cell-based assay for human P2X7 receptor is reported. We established permanent lines of HEK 293 cells expressing a high level of hP2X7 receptor. Functional activity of the hP2X7 receptor was confirmed by whole-cell patch recording of ATP-induced ion currents. Prolonged exposure to ATP resulted in death of the hP2X7-expressing REK 293 cells. and this cell death could be quantified. Two known P2X7 antagonists, PPADS and KN-62, blocked ATP-induced death in a concentration-dependent manner. Thus, this assay can be used to screen for new antagonists of hP2X7 receptors. © KSMCB 2006.
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약학대학 > 약학과 > Journal papers
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