Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김광호 | * |
dc.contributor.author | 이령아 | * |
dc.date.accessioned | 2016-08-28T12:08:31Z | - |
dc.date.available | 2016-08-28T12:08:31Z | - |
dc.date.issued | 2006 | * |
dc.identifier.issn | 1007-9327 | * |
dc.identifier.other | OAK-3596 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/219764 | - |
dc.description.abstract | Aim: To study whether hemoglobin could amplify colon cancer cell proliferation via reactive oxygen species (ROS) production. Methods: Colon cancer cell line HT-29 was grown in the conventional method using RPMI1640 media. The viability of the cells was measured using the colorimetric MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide] assay after adding hemoglobin. We determined reactive oxygen species levels to be indicators of oxidative stress in HT 29 cell lines with and without hemoglobin and/or 5-fluorouracil (5-FU), 5′-deoxy-5-fluorouridine (5-DFUR) using fluorometric dichlorofluorescin diacetate (DCFH-DA) assay. Results: Cellular proliferation was increased with hemoglobin in a concentration-dependent manner. A significant increment on ROS levels was found in HT 29 cells following hemoglobin incubation. The cytotoxic effects of 5-FU and 5-DFUR were significantly blunted by administration of hemoglobin. There was a slight increase of peroxiredoxin 1, superoxide dismutase 1 concentration according to different hemoglobin concentrations. Conclusion: Hemoglobin has a cellular proliferative effect on HT-29 colon cancer cell line by production of ROS. Also, hemoglobin abates cytotoxic effects of chemotherapeutic agents such as 5-FU and 5-DFUR. © 2006 The WJG Press. All rights reserved. | * |
dc.language | English | * |
dc.title | Hemoglobin induces colon cancer cell proliferation by release of reactive oxygen species | * |
dc.type | Article | * |
dc.relation.issue | 35 | * |
dc.relation.volume | 12 | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 5644 | * |
dc.relation.lastpage | 5650 | * |
dc.relation.journaltitle | World Journal of Gastroenterology | * |
dc.identifier.wosid | WOS:000241216000008 | * |
dc.identifier.scopusid | 2-s2.0-33750118400 | * |
dc.author.google | Lee R.-A. | * |
dc.author.google | Kim H.-A. | * |
dc.author.google | Kang B.-Y. | * |
dc.author.google | Kim K.-H. | * |
dc.contributor.scopusid | 김광호(57192983544) | * |
dc.contributor.scopusid | 이령아(12753883700) | * |
dc.date.modifydate | 20240118124654 | * |