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Selection of neural differentiation-specific genes by comparing profiles of random differentiation

Title
Selection of neural differentiation-specific genes by comparing profiles of random differentiation
Authors
Min S.L.Jun D.-H.Hwang C.-I.Seung S.P.Kang J.J.Park H.-S.Kim J.Ju H.K.Seo J.-S.Park W.-Y.
Ewha Authors
박승수이민수박현석
SCOPUS Author ID
박승수scopus; 이민수scopus; 박현석scopus
Issue Date
2006
Journal Title
Stem Cells
ISSN
1066-5099JCR Link
Citation
vol. 24, no. 8, pp. 1946 - 1955
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Differentiation of embryonic stem cells (ESCs) into neurons requires a high level of transcriptional regulation. To further understand the transcriptional regulation of neural differentiation of ESCs, we used oligonucleotide microarray to examine the gene expressions of the guided differentiation (GD) model for dopaminergic (DA) neurons from mouse ESCs. We also determined the gene expression profiles of the random differentiation (RD) model of mouse ESCs into embryoid bodies. From K-means clustering analysis using the expression patterns of the two models, most of the genes (1,282 of 1,884 genes [68.0%]) overlapped in their expression patterns. Six hundred twenty-two differentially expressed genes (DEGs) from the GD model by random variance F-test were classified by their critical molecular functions in neurogenesis and DNA replication (Gene Ontology analysis). However, 400 genes among GD-DEGs (64.3%) showed a high correlation with RD in Spearman's correlation analysis (Spearman's coefficient p s ≥ .6). The genes showing marginal correlation (-.4 < p s < .6) were present in the early stages of differentiation of both GD and RD, which were non-specific to brain development. Finally, we distinguished 66 GD-specific genes based on ps ≤ -.4, the molecular functions of which were related mainly to vesicle formation, neurogenesis, and transcription factors. From among these GD-specific genes, we confirmed the expression of Serpini1 and Rab33a in P19 differentiation models and adult brains. From these results, we identified the specific genes required for neural differentiation by comparing gene expressions of GD with RD; these would potentially be the highly specific candidate genes necessary for differentiation of DA neurons. ©AlphaMed Press.
DOI
10.1634/stemcells.2005-0325
Appears in Collections:
엘텍공과대학 > 컴퓨터공학과 > Journal papers
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