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Enhanced production of epothilones by carbon sources in Sorangium cellulosum

Title
Enhanced production of epothilones by carbon sources in Sorangium cellulosum
Authors
Park S.W.Choi S.H.Yoon Y.J.Lee D.H.Kim D.J.Kim J.-H.Lee Y.K.Choi G.J.Yeom I.-T.Sim S.J.
Ewha Authors
윤여준
SCOPUS Author ID
윤여준scopus
Issue Date
2006
Journal Title
Journal of Microbiology and Biotechnology
ISSN
1017-7825JCR Link
Citation
vol. 16, no. 4, pp. 519 - 523
Indexed
SCIE; SCOPUS; KCI WOS scopus
Abstract
To improve epothilones production and the ratio of epothilone B/A, carbon sources were investigated in flask culture of Sorangium cellulosum. Depending on the initial concentration, starch significantly enhanced cell growth, but the maximum epothilones productivity and the maximum epothilones production (0.3 mg/l · day and 2.6 mg/l at 15 g/l starch, respectively) were relatively low compared with cell growth. On the other hand, addition of glycerol did not stimulate cell growth, but epothilone production was increased from 2.81 mg/l to 7.59 mg/l. Addition of glycerol to culture medium resulted in more significant enhancement of the production of epothilone A, whereas epothilone B levels were relatively constant. Furthermore, when sodium propionate was added as a precursor of methylmalonyl-CoA, it resulted in increase of both total epothilones production and epothilone B/A resolution. Maximum epothilone A and B concentrations reached 10.9 mg/l and 8.58 mg/l, respectively, at 5 mM sodium propionate. © The Korean Society for Microbiology and Biotechnology.
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자연과학대학 > 화학·나노과학전공 > Journal papers
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