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TRPC4 is an essential component of the nonselective cation channel activated by muscarinic stimulation in mouse visceral smooth muscle cells

Title
TRPC4 is an essential component of the nonselective cation channel activated by muscarinic stimulation in mouse visceral smooth muscle cells
Authors
Lee K.P.Jun J.Y.Chang I.-Y.Suh S.-H.So I.Kim K.W.
Ewha Authors
서석효
SCOPUS Author ID
서석효scopus
Issue Date
2005
Journal Title
Molecules and Cells
ISSN
1016-8478JCR Link
Citation
vol. 20, no. 3, pp. 435 - 441
Indexed
SCI; SCIE; SCOPUS; KCI WOS scopus
Abstract
Classical transient receptor potential channels (TRPCs) are thought to be candidates for the nonselective cation channels (NSCCs) involved in pacemaker activity and its neuromodulation in murine stomach smooth muscle. We aimed to determine the role of TRPC4 in the formation of NSCCs and in the generation of slow waves. At a holding potential of -60 mV, 50 μM carbachol (CCh) induced INSCC of amplitude [500.8 ± 161.8 pA (n = 8)] at -60 mV in mouse gastric smooth muscle cells. We investigated the effects of commercially available antibodies to TRPC4 on recombinant TRPC4 expressed in HEK cells and CCh-induced NSCCs in gastric smooth muscle cells. TRPC4 currents in HEK cells were reduced from 1525.6 ±414.4 pA (n = 8) to 146.4 ± 83.3 pA (n = 10) by anti-TRPC4 antibody and INSCC amplitudes were reduced from 230.9 ± 36.3 pA (n = 15) to 49.8 ± 11.8 pA (n = 9). Furthermore, INScc in the gastric smooth muscle cells of TRPC4 knockout mice was only 34.4 ± 10.4 pA (n = 8) at -60 mV. However, slow waves were still present in the knockout mice. Our data suggest that TRPC4 is an essential component of the NSCC activated by muscarinic stimulation in the murine stomach. ©KSMCB 2005.
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의학전문대학원 > 의학과 > Journal papers
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