Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 허규정 | - |
dc.date.accessioned | 2016-08-28T11:08:58Z | - |
dc.date.available | 2016-08-28T11:08:58Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.other | OAK-2985 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/219630 | - |
dc.description.abstract | The insulin-mediated Ras/mitogen-activated protein (MAP) kinase cascade was examined in SK-N-BE(2) and PC12 cells, which can and cannot produce reactive oxygen species (ROS), respectively. Tyrosine phosphorylation of the insulin receptor and insulin receptor substrate 1 (IRS-1) was much lower in SK-N-BE(2) cells than in PC12 cells when the cells were treated with insulin. The insulin-mediated interaction of IRS-1 with Grb2 was observed in PC12 but not in SK-N-BE(2) cells. Moreover, the activity of extracellular-signal-related kinase (ERK) was much lower in SK-N-BE(2) than in PC12 cells when the cells were treated with insulin. Application of exogenous H2O2 caused increased tyrosine phosphorylation and Grb2 binding to IRS-1 in SK-N-BE(2) cells, while exposure to an H2O2 scavenger (N-acetylcysteine) or to a phophatidylinositol-3 kinase inhibitor (wortmannin), and expression of a dominant negative Rac1, decreased the activation of ERK in insulin-stimulated PC12 cells. These results indicate that the transient increase of ROS is needed to activate ERK in insulin-mediated signaling and that an inability to generate ROS is the reason for the insulin insensitivity of SK-N-BE(2) cells. ©KSMCB 2005. | - |
dc.language | English | - |
dc.title | Insulin cannot activate extracellular-signal-related kinase due to inability to generate reactive oxygen species in SK-N-BE(2) human neuroblastoma cells | - |
dc.type | Article | - |
dc.relation.issue | 2 | - |
dc.relation.volume | 20 | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.index | KCI | - |
dc.relation.startpage | 280 | - |
dc.relation.lastpage | 287 | - |
dc.relation.journaltitle | Molecules and Cells | - |
dc.identifier.wosid | WOS:000233001400017 | - |
dc.identifier.scopusid | 2-s2.0-33645224486 | - |
dc.author.google | Hwang J.-J. | - |
dc.author.google | Hur K.C. | - |
dc.contributor.scopusid | 허규정(7005230301) | - |
dc.date.modifydate | 20190301081000 | - |