Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 서주영 | * |
dc.contributor.author | 유경하 | * |
dc.contributor.author | 유은선 | * |
dc.contributor.author | 우소연 | * |
dc.date.accessioned | 2016-08-28T11:08:56Z | - |
dc.date.available | 2016-08-28T11:08:56Z | - |
dc.date.issued | 2005 | * |
dc.identifier.issn | 0888-0018 | * |
dc.identifier.other | OAK-2915 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/219611 | - |
dc.description.abstract | Arsenic trioxide has recently been shown to inhibit growth and induce apoptosis in a variety of hematologic malignancies, but very little is known about its effects on solid tumors and especially on neuroblastoma cells that have self-differentiating characteristics. To demonstrate the growth inhibition induced in neuroblastoma cells (the SH-SY5Y and SK-N-AS cell line) and acute promyelocytic leukemia cells (HL-60) by arsenic trioxide (As 2O 3), the viable cell numbers were counted after trypan blue staining. Apoptosis was assessed by the cell morphology, by flow cytometry with annexin-V staining, and by Western blot analysis for the apoptosis-relafed proteins (bcl-2 and PARP). To decide the dose for the clinical application of AS 2O 3, normal peripheral blood lymphocytes were also examined. The growth and survival of the SH-SY5Y and SK-N-AS cells were markedly inhibited by As 2O 3 treatment at a 3 μM concentration before the changes of the normal lymphocytes were observed. The apoptotic cells showed a shrunken cell nucleus, and an increase in the number and balloon-like swelling of the mitochondria at 72 h after the As 2O 3 was added. Apoptosis of the annexin-V-positive cell proportion in the neuroblastoma cell lines was increased with increasing the exposure time and the concentration of As 2O 3, just like the HL-60 cells. Bcl-2 downregulation and PARP degradation were also noted all the cell lines, but these changes were not statistically significant among the 3 cell lines. Taken together, these results indicate that As 2O 3 is an excellent candidate as a therapeutic agent for the treatment of neuroblastoma. Copyright © Taylor and Francis Inc. | * |
dc.language | English | * |
dc.title | Morphological and biochemical changes induced by arsenic trioxide in neuroblastoma cell lines | * |
dc.type | Article | * |
dc.relation.issue | 7 | * |
dc.relation.volume | 22 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 609 | * |
dc.relation.lastpage | 621 | * |
dc.relation.journaltitle | Pediatric Hematology and Oncology | * |
dc.identifier.doi | 10.1080/08880010500198897 | * |
dc.identifier.wosid | WOS:000232105200009 | * |
dc.identifier.scopusid | 2-s2.0-25444490454 | * |
dc.author.google | Ryu K.-H. | * |
dc.author.google | Woo S.-Y. | * |
dc.author.google | Lee M.-Y. | * |
dc.author.google | Jung Y.-J. | * |
dc.author.google | Yoo E.-S. | * |
dc.author.google | Seoh J.-Y. | * |
dc.author.google | Kie J.-H. | * |
dc.author.google | Shin H.-Y. | * |
dc.author.google | Ahn H.-S. | * |
dc.contributor.scopusid | 서주영(6603709174;57209001625) | * |
dc.contributor.scopusid | 유경하(14038236200) | * |
dc.contributor.scopusid | 유은선(20936704200) | * |
dc.contributor.scopusid | 우소연(7402853365) | * |
dc.date.modifydate | 20240118130224 | * |