Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 홍영숙 | - |
dc.date.accessioned | 2016-08-28T11:08:53Z | - |
dc.date.available | 2016-08-28T11:08:53Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 0008-5472 | - |
dc.identifier.other | OAK-2792 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/219583 | - |
dc.description.abstract | Bcl-2 is involved in the progression of human malignancies, but the precise role and mechanism of Bcl-2 for tumor invasion and metastasis remains unclear. In this study, we have investigated the role and mechanism of Bcl-2 on tumor cell invasion and metastasis by using Bcl-2 overexpressing non-small cell lung cancer cells. Matrix metalloproteinases (MMPs) are important proteins involved in the processes of tumor invasion and metastasis. In vitro Matrigel invasion assays showed that Bcl-2 overexpression increased tumor cell invasion by 15-fold. Moreover, Bcl-2 overexpression enhanced in vivo lung metastasis by 4-fold. Consistent with its effect on invasion and metastasis, Bcl-2 overexpression induced not only MMP-2 mRNA and its protein expression, but this also activated the pro-MMP-2 protein to its active form. To explore the induction mechanism of MMP-2 by Bcl-2, we investigated the effects of Bcl-2 overexpression on MMP-2 transcriptional regulation. Nuclear run-on assays showed a 6-fold increase in the transcription rate of MMP-2 mRNA in the Bcl-2 transfectants (H157/Bcl-2) compared with that of the H157/vector control cells (H157/C). Overexpression of Bcl-2 induced the nuclear transcription factor activator protein 1 family, including the c-Jun, JunD, c-Fos, FosB, and Fra-1 proteins. Reporter assays combined with deletion mutagenesis analysis and gel shift assays showed the involvement of activator protein 1 in the activation of MMP-2 promoter activity by Bcl-2. Taken together, we have shown that Bcl-2 promotes tumor invasion and lung metastasis by inducing MMP-2 gene expression through the combined action of transcriptional and posttranslational mechanisms. ©2005 American Association for Cancer Research. | - |
dc.language | English | - |
dc.title | Bcl-2 promotes invasion and lung metastasis by inducing matrix metalloproteinase-2 | - |
dc.type | Article | - |
dc.relation.issue | 13 | - |
dc.relation.volume | 65 | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 5554 | - |
dc.relation.lastpage | 5560 | - |
dc.relation.journaltitle | Cancer Research | - |
dc.identifier.doi | 10.1158/0008-5472.CAN-04-4570 | - |
dc.identifier.wosid | WOS:000230165000013 | - |
dc.identifier.scopusid | 2-s2.0-21344473197 | - |
dc.author.google | Choi J. | - |
dc.author.google | Choi K. | - |
dc.author.google | Benveniste E.N. | - |
dc.author.google | Hong Y.-S. | - |
dc.author.google | Lee J.-H. | - |
dc.author.google | Kim J. | - |
dc.author.google | Park K. | - |
dc.contributor.scopusid | 홍영숙(57218968598) | - |
dc.date.modifydate | 20180329105800 | - |