The role of a second protein (Des VIII) in glycosylation for the biosynthesis of hybrid macrolide antibiotics in Streptomyces venezuelae

Abstract

The function of the desVIII gene in the pikromycin producer Streptomyces venezuelae was characterized by gene deletion and complementation analysis. In addition to the DesVII glycosyltransferase, the desVIII gene that has previously been suggested to be required for the incorporation of endogenous. deoxysugar, TDP-D-desosamine, into the aglycone of pikromycin is also required for the transfer of exogenous deoxysugars, TDP-D-quinovose and TDP-D-olivose. © The Korean Society for Microbiology and Biotechnology.