Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 오구택 | * |
dc.date.accessioned | 2016-08-28T11:08:47Z | - |
dc.date.available | 2016-08-28T11:08:47Z | - |
dc.date.issued | 2004 | * |
dc.identifier.issn | 1016-8478 | * |
dc.identifier.other | OAK-2524 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/219514 | - |
dc.description.abstract | We have developed a method for rapid activity staining of proteins with glutathione peroxidase (GPx) activity after 2-D gel electrophoresis. After separating proteins extracted from yeast, or mouse red blood cells, by two-dimensional gel electrophoresis, SDS was removed and the gel was submerged in a Tris-HCl buffer containing glutathione and hydrogen peroxide, followed by incubation with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and phenazine methosulfate (PMS). After this proteins with GPx activity appeared as clear zones on a purple background. This relatively simple activity staining method could be useful for rapid screening of proteins with GPx activity in cell extracts. ©KSMCB 2004. | * |
dc.language | English | * |
dc.title | Activity staining of glutathione peroxidase after two-dimensional gel electrophoresis | * |
dc.type | Article | * |
dc.relation.issue | 3 | * |
dc.relation.volume | 18 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.index | KCI | * |
dc.relation.startpage | 369 | * |
dc.relation.lastpage | 373 | * |
dc.relation.journaltitle | Molecules and Cells | * |
dc.identifier.wosid | WOS:000226042600014 | * |
dc.identifier.scopusid | 2-s2.0-14544304561 | * |
dc.author.google | Kho C.W. | * |
dc.author.google | Park S.G. | * |
dc.author.google | Lee D.H. | * |
dc.author.google | Cho S. | * |
dc.author.google | Oh G.T. | * |
dc.author.google | Kang S. | * |
dc.author.google | Park B.C. | * |
dc.contributor.scopusid | 오구택(7007056663) | * |
dc.date.modifydate | 20240123094756 | * |