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Activity staining of glutathione peroxidase after two-dimensional gel electrophoresis

Title
Activity staining of glutathione peroxidase after two-dimensional gel electrophoresis
Authors
Kho C.W.Park S.G.Lee D.H.Cho S.Oh G.T.Kang S.Park B.C.
Ewha Authors
오구택
SCOPUS Author ID
오구택scopus
Issue Date
2004
Journal Title
Molecules and Cells
ISSN
1016-8478JCR Link
Citation
vol. 18, no. 3, pp. 369 - 373
Indexed
SCI; SCIE; SCOPUS; KCI WOS scopus
Abstract
We have developed a method for rapid activity staining of proteins with glutathione peroxidase (GPx) activity after 2-D gel electrophoresis. After separating proteins extracted from yeast, or mouse red blood cells, by two-dimensional gel electrophoresis, SDS was removed and the gel was submerged in a Tris-HCl buffer containing glutathione and hydrogen peroxide, followed by incubation with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and phenazine methosulfate (PMS). After this proteins with GPx activity appeared as clear zones on a purple background. This relatively simple activity staining method could be useful for rapid screening of proteins with GPx activity in cell extracts. ©KSMCB 2004.
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자연과학대학 > 생명과학전공 > Journal papers
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