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MicroRNA genes are transcribed by RNA polymerase II

Title
MicroRNA genes are transcribed by RNA polymerase II
Authors
Lee Y.Kim M.Han J.Yeom K.-H.Lee S.Baek S.H.Kim V.N.
Ewha Authors
이상혁
SCOPUS Author ID
이상혁scopus
Issue Date
2004
Journal Title
EMBO Journal
ISSN
0261-4189JCR Link
Citation
vol. 23, no. 20, pp. 4051 - 4060
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
MicroRNAs (miRNAs) constitute a large family of noncoding RNAs that function as guide molecules in diverse gene silencing pathways. Current efforts are focused on the regulatory function of miRNAs, while little is known about how these unusual genes themselves are regulated. Here we present the first direct evidence that miRNA genes are transcribed by RNA polymerase II (pol II). The primary miRNA transcripts (pri-miRNAs) contain cap structures as well as poly (A) tails, which are the unique properties of class II gene transcripts. The treatment of human cells with α-amanitin decreased the level of pri-miRNAs at a concentration that selectively inhibits pol II activity. Furthermore, chromatin immunoprecipitation analyses show that pol II is physically associated with a miRNA promoter. We also describe, for the first time, the detailed structure of a miRNA gene by determining the promoter and the terminator of mir-23a∼27a∼24-2. These data indicate that pol II is the main, if not the only, RNA polymerase for miRNA gene transcription. Our study offers a basis for understanding the structure and regulation of miRNA genes.
DOI
10.1038/sj.emboj.7600385
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자연과학대학 > 생명과학전공 > Journal papers
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