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Transcriptional activation of JC virus early promoter by phorbol ester and interleukin-1β: Critical role of nuclear factor-1

Title
Transcriptional activation of JC virus early promoter by phorbol ester and interleukin-1β: Critical role of nuclear factor-1
Authors
Kim S.-Y.Choi E.-C.Woo Jo Y.Henson J.W.Kim H.-S.
Ewha Authors
김희선
SCOPUS Author ID
김희선scopus
Issue Date
2004
Journal Title
Virology
ISSN
0042-6822JCR Link
Citation
vol. 327, no. 1, pp. 60 - 69
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
JC virus causes the fatal demyelinating disease, progressive multifocal leukoencephalopathy (PML) under immunosuppressive states such as AIDS. During the pathogenesis of AIDS, HIV-infected microglia secrete cytokines including interleukin-1 and tumor necrosis factor-α (TNF-α), which affect neuronal cells resulting in dysfunction of the CNS. We hypothesized that extracellular stimuli released from HIV-infected microglia may reactivate JC virus by affecting neighboring oligodendrocytes. In the present study, we found that phorbol myristate acetate (PMA) and interleukin-1β (IL-1β) dramatically increased JC virus transcription in glial cells. Site-directed mutagenesis and gel shift analyses revealed that PMA and IL-1β strongly induced nuclear factor-1 (NF-1) binding to the JC virus enhancer region, increasing transcriptional activity of the viral early promoter. Additionally, we demonstrated that protein kinase C (PKC) pathways were involved in the PMA/IL-1β-mediated up-regulation of the JC virus early promoter. These findings may represent one of the possible mechanisms for higher incidence of PML among AIDS patients. © 2004 Elsevier Inc. All rights reserved.
DOI
10.1016/j.virol.2004.06.021
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의학전문대학원 > 의학과 > Journal papers
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