Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 박성수 | - |
dc.date.accessioned | 2016-08-28T11:08:43Z | - |
dc.date.available | 2016-08-28T11:08:43Z | - |
dc.date.issued | 2004 | - |
dc.identifier.issn | 0022-1147 | - |
dc.identifier.other | OAK-2320 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/219463 | - |
dc.description.abstract | We report the development of automated flourometric immunoassay for the detection of Escherichia coli O157:H7, using antibody-directed liposomes (immunoliposomes) encapsulating fluorophore as an analytical reagent. Thiolated antibodies (anti-E. coli O157:H7) were coupled to malemide-tagged liposomes encapsulating dye. To automate the assay, a fluorescence plate reader was included in the assay system to detect fluorophore released from lysed liposomes in a microplate. The detection limit of the current assay with pure cultures of the serotype was about 104 colony-forming units (CFU)/mL. The assay can detect E. coli O157 in ground beef samples inoculated with as few as 0.8 CFU/mL after a 12-h enrichment. These results demonstrate the feasibility of using fluorophore-encapsulated immunoliposomes in a microtiter plate for the rapid and automated detection of molecules with multivalent antigenic sites. | - |
dc.language | English | - |
dc.title | Immunoliposomes sandwich fluorometric assay (ILSF) for detection of Escherichia coli O157:H7 | - |
dc.type | Article | - |
dc.relation.issue | 6 | - |
dc.relation.volume | 69 | - |
dc.relation.index | SCI | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | M151 | - |
dc.relation.lastpage | M156 | - |
dc.relation.journaltitle | Journal of Food Science | - |
dc.identifier.wosid | WOS:000223489600021 | - |
dc.identifier.scopusid | 2-s2.0-4143114591 | - |
dc.author.google | Park S. | - |
dc.author.google | Oh S. | - |
dc.author.google | Durst R.A. | - |
dc.contributor.scopusid | 박성수(9275920900;41262153700) | - |
dc.date.modifydate | 20230627101106 | - |