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Characteristics of chitosanases from Aspergillus fumigatus KB-1.

Title
Characteristics of chitosanases from Aspergillus fumigatus KB-1.
Authors
Eom T.K.Lee K.M.
Ewha Authors
이강만
SCOPUS Author ID
이강만scopus
Issue Date
2003
Journal Title
Archives of pharmacal research
ISSN
0253-6269JCR Link
Citation
vol. 26, no. 12, pp. 1036 - 1041
Indexed
SCIE; SCOPUS; KCI WOS scopus
Abstract
Two chitosanases produced by Aspergillus fumigatus KB-1 were purified by ion exchange and size exclusion chromatographies. Molecular weights of chitosanases were 111.23 kDa (chitosanase I) and 23.38 kDa (chitosanase II). The N-terminal amino acid sequence of chitosanase II was determined as follows: YNLPNNLKQIYDKHKGKXSXVLAKGFTN. The optimum pH of the chitosanase I and II was 6.5 and 5.5, respectively. The optimum temperatures were 60 degrees C for chitosanase land 70 degrees C for chitosanase II. Hydrolysis products of two chitosanases were analyzed by HPLC and GPC. Chitosanase I hydrolyzed substrate to glucosamine. Chitosanase II produced chitooligosaccharides.
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약학대학 > 약학과 > Journal papers
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