Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김형래 | * |
dc.date.accessioned | 2016-08-28T11:08:32Z | - |
dc.date.available | 2016-08-28T11:08:32Z | - |
dc.date.issued | 2003 | * |
dc.identifier.issn | 1226-3613 | * |
dc.identifier.other | OAK-1741 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/219344 | - |
dc.description.abstract | For the comprehensive analysis of transcript expression, the array-based hybridization analysis and the serial analysis of gene expression (SAGE) are commonly used platforms. The SAGE is based on a high-throughput sequencing of ditags derived from the transcript. DNA microarrays are a powerful tool for monitoring thousands of transcripts simultaneously, whereas the Genechip (Affimatrix microarray) technology is based on the hybridization of a single probe or other manufacturer's microarrays (cDNA- or oligonucleotide-microarray) procedures include the competitive hybridization of two probes. In this study, the quantitative accuracy of expression using oligonucleotide-microarray was determined by comparing data set from the SAGE. In previous study the microSAGE was performed for the megakaryocytes and nonmegakaryocytes derived from human cord blood CD34+ cells by ex vivo expansion using thrombopoietin, and a total of 38,909 tags representing 8,976 unique genes were obtained. On the identical RNA, expression profiling was also carried out using oligonucleotide-microarray (MAGIC II 10K chip, Macrogen). The most frequently expressed genes in human megakaryocytes were identified as platelet factor 1 followed by annexin Al, ribosomal protein S23. The majority of the 50 most highly expressed genes in the CD34+-derived megakaryocytes were those involved in protein synthesis, e.g., ribosomal proteins. The expression level through the single channel of oligonucleotide-microarray and SAGE have a fairly good correlation in terms of absolute analyses and that the correlation is higher for the genes with higher expression levels. | * |
dc.language | English | * |
dc.title | Comparison of oligonucleotide-microarray and serial analysis of gene expression (SAGE) in transcript profiling analysis of megakaryocytes derived from CD34+ cells | * |
dc.type | Article | * |
dc.relation.issue | 5 | * |
dc.relation.volume | 35 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.index | KCI | * |
dc.relation.startpage | 460 | * |
dc.relation.lastpage | 466 | * |
dc.relation.journaltitle | Experimental and Molecular Medicine | * |
dc.identifier.wosid | WOS:000186884600017 | * |
dc.identifier.scopusid | 2-s2.0-0344873702 | * |
dc.author.google | Kim H.-L. | * |
dc.contributor.scopusid | 김형래(57202558385;57219111690;57567109600) | * |
dc.date.modifydate | 20240118123830 | * |