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Triacylglycerol, 1-palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol isolated from bovine udder and its synthetic enantiomer can potentiate the mitogenic activity for mouse peritoneal macrophages

Title
Triacylglycerol, 1-palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol isolated from bovine udder and its synthetic enantiomer can potentiate the mitogenic activity for mouse peritoneal macrophages
Authors
Suh J.S.Kwon J.Eun J.S.Lee Y.J.Limb J.K.Ko S.Y.Han S.Y.Bae Y.S.Jhon G.J.
Ewha Authors
전길자배윤수고수영한소엽임진경
SCOPUS Author ID
전길자scopus; 배윤수scopus; 고수영scopus; 한소엽scopus; 임진경scopus
Issue Date
2003
Journal Title
Cellular Physiology and Biochemistry
ISSN
1015-8987JCR Link
Citation
vol. 13, no. 6, pp. 415 - 422
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
A factor stimulating a mitogenic activity of peritoneal macrophages is purified from bovine udder. It is identified as a triglyceride, 1-palmitoyl-2-linoleoyl-3-acetyl-rac-glycerol (rac-MADG). In this study, its enantiomers, R-(+)-and S-(-)-1-palmitoyl-2-linoleoyl-3-acetylglycerol (R-(+)-MADG, S-(-)-MADG) are synthesized. Among them, R-(+)-MADG enantiomer turns out to increase a mitogenic activity in mouse peritoneal macrophages. Also, (S)-(-)-MADG shows a low mitogenic activity. Treatment of a macrophage with R-(+)-MADG increases reactive oxygen species (ROS). Furthermore, treatment of macrophages with antioxidant, N-acetyl-L-cysteine (NAC), suppresses the R-(+)-MADG-dependent macrophage proliferation. Results show that the generation of ROS induces in R-(+)-MADG-dependent cell signaling. Treatment of a macrophage with R-(+)-MADG increases the activity of protein kinase C (PKC). Treatment of macrophages with calphostin C inhibits R-(+)-MADG-induced macrophage proliferation. Results suggest that R-(+)-MADG enhances the activity of protein kinase C (PKC) and stimulates the macrophage growth. In conclusions, R-(+)-MADG accelerates the production of ROS and increases the activity of PKC to eventually stimulate macrophage cell growth. The existence of rac-MADG in bovine udder and milk provides passive protection for the neonate and immunostimulatory capabilities. Copyright © 2003 S. Karger AG, Basel.
DOI
10.1159/000075129
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자연과학대학 > 화학·나노과학전공 > Journal papers
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