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Induction of the differentiation of HL-60 promyelocytic leukemia cells by L-ascorbic acid

Title
Induction of the differentiation of HL-60 promyelocytic leukemia cells by L-ascorbic acid
Authors
Kang H.K.Suh J.H.Lee J.J.Yoon S.H.Hyun J.W.Choi S.W.Choi J.Y.Ryu K.H.Chung M.H.
Ewha Authors
유경하
SCOPUS Author ID
유경하scopus
Issue Date
2003
Journal Title
Free Radical Research
ISSN
1071-5762JCR Link
Citation
vol. 37, no. 7, pp. 773 - 779
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
The present study was undertaken to examine the effect of L-ascorbic acid (LAA) on the growth of HL-60 promyelocytic leukemia cells, besides induction of apoptosis. LAA (≥ 10 -4 M) was found to markedly inhibit the proliferation of HL-60 in liquid culture and clonogenicity in semisolid culture. Moreover, LAA-treated HL-60 showed activity to produce chemiluminescence and expressed CD 66b cell surface antigens, indicating that LAA induces the differentiation of HL-60 mainly into granulocytes. The results are supported by morphological changes of LAA-treated HL-60 into segmented neutrophils. Therefore, the inhibitory effect of LAA on the growth of HL-60 cells seems to arise from the induction of differentiation. To assess the potential role of LAA, cells were exposed to oxygen radical scavengers in the absence or presence of LAA. Catalase abolished and superoxide dismutase promoted LAA-induced differentiation of HL-60. Thus, H 2O 2 produced as a result of LAA treatment seems to play a major role in induction of HL-60 differentiation.
DOI
10.1080/1071576031000103078
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
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