Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 서주영 | * |
dc.contributor.author | 유경하 | * |
dc.date.accessioned | 2016-08-28T11:08:19Z | - |
dc.date.available | 2016-08-28T11:08:19Z | - |
dc.date.issued | 2003 | * |
dc.identifier.issn | 1011-8934 | * |
dc.identifier.other | OAK-1454 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/219202 | - |
dc.description.abstract | Bcr-abl antisense oligodeoxynucleotides (AS-ODNs) have provided evidence of an antileukemia effect when tested in vitro against Philadelphia-positive cells. In order to investigate the efficacy of AS-ODNs as purging agents in chronic myeloid leukemia (CML) patients, K562 cells, a human CML cell line, were treated in vitro with various types of AS-ODNs and interferon-α. Cells were treated in vitro for 0 and 36 hr with 40 μg/mL of AS-ODNs, respectively, and incubated at 37°C for 36 hr. Cytotoxic effects were measured by counting the number of viable cells as well as by MTT test. Clonogenic activities were evaluated by methylcellulose culture for 2 weeks. The effects of purging agents on the rearrangement of bcr-abl gene were evaluated by RT-PCR. AS-ODNs inhibited the proliferation of K562 cells with time in cell count assay and MTT test. AS-ODNs were superior to INF-α in inhibiting clonogenic activity (recovery rate; 26.3% vs 64.0%). After incubation with bcr-abl AS-ODNs primers and mRNA isolated from K562 cells, positive bands were abolished, especially of b3a2 type and phosphorothioate type. Our results suggest that AS-ODNs mediated purging may be one of the efficient methods and that autograft may be an alternative treatment for allograft in high-risk group patients of CML if they do not have a stem cell donor. | * |
dc.language | English | * |
dc.title | Myeloablative Treatment Supported by Autologous Stem Cell Infusion with Neuroblastoma | * |
dc.type | Article | * |
dc.relation.issue | 2 | * |
dc.relation.volume | 18 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.index | KCI | * |
dc.relation.startpage | 184 | * |
dc.relation.lastpage | 190 | * |
dc.relation.journaltitle | Journal of Korean Medical Science | * |
dc.identifier.wosid | WOS:000182615800007 | * |
dc.identifier.scopusid | 2-s2.0-0346256527 | * |
dc.author.google | Ryu K.H. | * |
dc.author.google | Seoh J.Y. | * |
dc.author.google | Jang P.S. | * |
dc.author.google | Kim C.W. | * |
dc.author.google | Koh S.H. | * |
dc.author.google | Shin H.Y. | * |
dc.author.google | Ahn H.S. | * |
dc.contributor.scopusid | 서주영(6603709174;57209001625) | * |
dc.contributor.scopusid | 유경하(14038236200) | * |
dc.date.modifydate | 20240118130224 | * |