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Interacting domain between yeast chitin synthase 3 and chitin synthase 4 is involved in biogenesis of chitin ring, but not for cell wall chitin

Title
Interacting domain between yeast chitin synthase 3 and chitin synthase 4 is involved in biogenesis of chitin ring, but not for cell wall chitin
Authors
Choi S.-J.Park N.-H.Park H.-S.Park M.-H.Woo J.-E.Choi W.
Ewha Authors
최원자
SCOPUS Author ID
최원자scopus
Issue Date
2003
Journal Title
Journal of Microbiology and Biotechnology
ISSN
1017-7825JCR Link
Citation
vol. 13, no. 2, pp. 263 - 268
Indexed
SCIE; SCOPUS; KCI WOS scopus
Abstract
Recently, we identified a domain, termed MIRC3-4, for the protein-protein interaction between yeast chitin synthase 3 (CHS3) and chitin synthase 4 (CHS4). In this study, the functional roles of MIRC3-4 were examined at the G1 phase and cytokinesis of the cell cycle by Calcofluor staining and FISH. Some mutations in MIRC3-4 resulted in disappearance of the chitin ring in the early G1 phase, but did not affect chitin synthesis in the cell wall at cytokinesis. The chitin distribution in chs4 mutant cells indicated that CHS4 was involved in the synthesis of chitin ring in the G1 phase and in the synthesis of cell wall chitin after cytokinesis, suggesting that Chs4p regulates chitin synthase 3 activity differently in G1 and cytokinesis. Absence of the chitin ring could be caused either by delocalization of Chs3p to the bud-neck or by improper interaction with Chs4p. When mutant cells were immunostained with a Chs3p-specific antibody to discriminate between these two alternatives, the mutated Chs3p was found to localize to the bud-neck in all MIRC3-4 mutants. These results strongly indicate that Chs4p regulates Chs3p as an activator but not a recruiter.
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자연과학대학 > 생명과학전공 > Journal papers
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