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In vitro differentiation of natural killer T cells from human cord blood CD34+ cells

Title
In vitro differentiation of natural killer T cells from human cord blood CD34+ cells
Authors
Woo S.-Y.Jung Y.-J.Ryu K.-H.Park H.-Y.Kie J.-H.Im S.-A.Chung W.-S.Han H.-S.Seoh J.-Y.
Ewha Authors
박혜영서주영정화순유경하우소연
SCOPUS Author ID
박혜영scopus; 서주영scopus; 정화순scopus; 유경하scopus; 우소연scopus
Issue Date
2003
Journal Title
British Journal of Haematology
ISSN
0007-1048JCR Link
Citation
vol. 121, no. 1, pp. 148 - 156
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Natural killer T (NKT) cells are involved in innate immune defence and also in the regulation of adaptive immune responses. However, the development of NKT cells in vitro has not been fully characterized and culture conditions have not been fully optimized. In the present study, we found that an NKT cell fraction developed during the in vitro culture of cord blood (CB) CD34+ cells, and this was subsequently characterized both phenotypically and morphologically. CD34+ cells purified from 10 human CB were cultured in the presence of several cytokines and analysed by flow cytometry, light microscopy and electron microscopy. The NKT cell fraction, defined phenotypically (CD3+CD16+CD56+CD94+) as expressing the invariant T-cell receptor Vα24 and Vβ11, appeared in the CD56hi fractions. Intracytoplasmic staining demonstrated that interferon-γ and interleukin 4 (IL-4) were detected in the CD56hi fractions. IL-15 was essential and, in combination with either flt3-1igand (FL) or stem cell factor (SCF), was sufficient to induce the development of NKT cells. The phenotype of the NKT cell fraction was CD45RO+CD45RA- and CD4+CD8α+. Morphologically, they were very large, with either round or oval nuclei, moderately condensed chromatins, voluminous weakly basophilic cytoplasm and various cytoplasmic granules such as dense core granules, multivesicular bodies, and intermediate form granules. When CD34+ cells purified from bone marrow (BM) were compared with those from CB, the latter were consistently more efficient at generating CD56hi NKT cell fractions. In conclusion, IL-15 in combination with EL and/or SCF can induce the differentiation of NKT cells from human CB CD34+ cells.
DOI
10.1046/j.1365-2141.2003.04230.x
Appears in Collections:
의과대학 > 의학과 > Journal papers
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