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Changes in phosphorylation of 50 and 53 kDa soluble proteins in graviresponding oat (Avena sativa) shoots

Title
Changes in phosphorylation of 50 and 53 kDa soluble proteins in graviresponding oat (Avena sativa) shoots
Authors
Chang S.C.Cho M.H.Kim S.-K.Lee J.S.Kirakosyan A.Kaufman P.B.
Ewha Authors
이준승
SCOPUS Author ID
이준승scopusscopus
Issue Date
2003
Journal Title
Journal of Experimental Botany
ISSN
0022-0957JCR Link
Citation
vol. 54, no. 384, pp. 1013 - 1022
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
The present work indicates that phosphorylation of a 50 kDa soluble protein is involved in the gravitropic response in graviresponsive pulvini of oat (Avena sativa) stems. This 50 kDa protein shows a differential pattern of phosphorylation between lower and upper halves of pulvini both in vivo and in vitro. The differential phosphorylation of this protein is detected only when stem segments are gravistimulated for short and long time periods. The differential phosphorylation of the 50 kDa protein occurs as early as 5 min after the initiation of gravistimulation. This corresponds closely to the presentation time of 5.2 min. This differential phosphorylation pattern was changed by treatments with cycloheximide, implying that a newly-synthesized protein is involved in the differential phosphorylation during the gravitropic response. An autophosphorylation experiment shows that the 50 kDa protein has kinase activity. The phosphorylation patterns of a 53 kDa protein were similar to those of the 50 kDa protein, but were only expressed in vitro. These findings indicate that the differential phosphorylation of the 50 (and 53 kDa) soluble proteins in graviresponding oat shoots may be an important component of the gravity signal transduction pathway.
DOI
10.1093/jxb/erg104
Appears in Collections:
자연과학대학 > 생명과학전공 > Journal papers
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