View : 13 Download: 0

Iron promotes the survival and neurite extension of serum-starved PC12 cells in the presence of NGF by enhancing cell attachment

Title
Iron promotes the survival and neurite extension of serum-starved PC12 cells in the presence of NGF by enhancing cell attachment
Authors
Hong J.-H.Noh K.-M.Yoo Y.-E.Choi S.-Y.Park S.-Y.Kim Y.-H.Chung J.-M.
Ewha Authors
정준모
SCOPUS Author ID
정준모scopus
Issue Date
2003
Journal Title
Molecules and Cells
ISSN
1016-8478JCR Link
Citation
vol. 15, no. 1, pp. 10 - 19
Indexed
SCI; SCIE; SCOPUS; KCI WOS scopus
Abstract
Delayed death of serum-starved PC12 cells on a poly-L-lysine (PLL) matrix was observed, even in the presence of NGF. NGF blocked the apoptotic death of attached but not detached cells, which suggests that delayed death may be related to cell detachment from the PLL matrix. Iron selectively blocked this anoikis-like death by increasing cell attachment. Interestingly, the addition of > 10 μM FeCl2 to the culture medium generated gelatinous iron precipitates, and the removal of the precipitates abolished the iron effect. Attachment experiments using poly-HEMA supported the role of iron precipitates on cell-to-matrix adhesion. The expression of integrin β1, neither N-cadherin nor α/β-catenin, was also significantly increased by iron. In addition to its effect on cell viability, iron promoted the outgrowth of neuntes. Our results collectively indicate that iron functions as a necessary co-element for NGF by enhancing cell attachment, survival, and neurite extension. ©KSMCB 2003.
Appears in Collections:
자연과학대학 > 생명과학전공 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE