Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 서주영 | - |
dc.date.accessioned | 2016-08-28T11:08:11Z | - |
dc.date.available | 2016-08-28T11:08:11Z | - |
dc.date.issued | 2002 | - |
dc.identifier.issn | 1016-8478 | - |
dc.identifier.other | OAK-1297 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/219126 | - |
dc.description.abstract | Although umbilical cord blood is increasingly being used in allogeneic marrow transplantation, delayed platelet engraftment is often a concern for cord blood transplant recipients. We evaluated the potential of ex vivo expansion and clonality in CD34+ cells separated from a bone marrow source, and cord blood, in a serum-free Media. The CD34+ cells, selected from bone marrow (BM) and umbilical cord blood (CB), were expanded with hematopoietic growth factors. They were then cultured for burst-forming units of erythrocytes (BFU-E), colony-forming units of granulocytes and monocytes (CFU-GM) and colony-forming units of megakaryocytes (CFU-Mk) at days 0, 4, 7, and 14 under the combination of growth factors, with cell counts. The cytokines included the recombinant human megakaryocyte growth and development (100 ng/ml), interleukin-3 (10 ng/ml), stem cell factor (100 ng/ml), flt-3 ligand (50 ng/ml) and interleukin-11 (200 ng/ml). The CB-selected CD34+ cells showed significantly higher total cell expansion than those from the BM at day 7 (3.0 fold increase than BM), day 14 (2.4 fold), and day 17 (2.6 fold). The colony count of the BFU-E/CFU-E per CD34+ cell at day 0 was 0.14 ± 0.023 in the CB, which was significantly higher than 0.071 ± 0.015 in the BM. The CB-selected CD34+ cells produced more BFU-E colonies than the BM on culture days 4, 7, and 14. The BFU-E colonies from the CB cells increased markedly on culture days 4 and 7, with a 4-fold increase at day 14. The colony count of the CFU-Mk per CD34+ cell at day 0 was 0.047 ± 0.011 in the CB-selected CD34+ cells cultures, which was higher than the 0.026 ± 0.014 in the BM. The CB-selected CD34+ cells produced more CFU-Mk colonies than the BM on culture days 4, 7 and 14. In conclusion, the ex vivo expansion of the CB cells may be very promising in producing total cellular expansion, CFU-Mk and BFU-E compared with BM, especially at day 7. The ex vivo expansion of the CB may have rationale in making an ex vivo culture for 7 to 14 d. ©KSMCB 2002. | - |
dc.language | English | - |
dc.title | Ex vivo expansion and clonality of CD34+ selected cells from bone marrow and cord blood in a serum-free media | - |
dc.type | Article | - |
dc.relation.issue | 3 | - |
dc.relation.volume | 14 | - |
dc.relation.index | SCI | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.index | KCI | - |
dc.relation.startpage | 367 | - |
dc.relation.lastpage | 373 | - |
dc.relation.journaltitle | Molecules and Cells | - |
dc.identifier.wosid | WOS:000180215300007 | - |
dc.identifier.scopusid | 2-s2.0-0037637673 | - |
dc.author.google | Kim S.-K. | - |
dc.author.google | Koh S.-K. | - |
dc.author.google | Song S.U. | - |
dc.author.google | Shin S.-H. | - |
dc.author.google | Choi G.-S. | - |
dc.author.google | Kim W.-C. | - |
dc.author.google | Lee M.-H. | - |
dc.author.google | Seoh J.Y. | - |
dc.author.google | Park S.K. | - |
dc.author.google | Fraser J.K. | - |
dc.contributor.scopusid | 서주영(6603709174;57209001625) | - |
dc.date.modifydate | 20230210140937 | - |