View : 13 Download: 0

Changes of GABAA receptor binding and subunit mRNA level in rat brain by infusion of NOS inhibitor

Title
Changes of GABAA receptor binding and subunit mRNA level in rat brain by infusion of NOS inhibitor
Authors
Kim Y.Oh S.
Ewha Authors
오세관
SCOPUS Author ID
오세관scopus
Issue Date
2002
Journal Title
Brain Research
ISSN
0006-8993JCR Link
Citation
vol. 952, no. 2, pp. 246 - 256
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
In the present study, we have investigated the effects of prolonged inhibition of nitric oxide synthase (NOS) by infusion of NOS inhibitor, L-nitroarginine, to examine the pentobarbital-induced sleep, modulation of GABAA receptor binding, and GABAA receptor subunit mRNA level in rat brain. Pre-treatment with L-nitroarginine 30 min before pentobarbital treatment (60 mg/kg, i.p.) significantly increased the duration of sleep in rats. However, the duration of pentobarbital-induced sleep was shortened by the prolonged infusion of L-nitroarginine into ventricle. We have investigated the effect of NOS inhibitor on GABAA receptor binding characteristics in discrete areas of brain regions by using autoradiographic and in situ hybridization techniques. Rats were infused with L-nitroarginine (10, 100 pmol/10 μl/h, i.c.v.) for 7 days, through pre-implanted cannula by osmotic minipumps. The levels of [3H]muscimol and [3H]flunitrazepam binding were markedly elevated in almost all of brain regions including cortex, caudate putamen, thalamus, hippocampus, and cerebellum. However, there was no change in the level of [35S]TBPS binding. The levels of β2-subunit were elevated in the cortex, brainstem, and cerebellar granule layers. By contrast, the levels of β3-subunit were significantly decreased in the cortex, hippocampus, and cerebellar granule layers in L-nitroarginine-infused rats. Following L-nitroarginine treatment, the levels of α6- and δ-subunits which were strictly localized to the cerebellum, were not changed in the cerebellar granule layer. These results show that the prolonged inhibition of NOS by L-nitroarginine-infusion markedly elevates [3H]muscimol and [3H]flunitrazepam binding throughout the brain, and alters GABAA receptor subunit mRNA levels in different directions. Chronic inhibition of NO generation has differential effects on the various expressions of GABAA receptor subunits. These suggest the involvement of different regulatory mechanisms for the NO-induced expression of GABAA receptor. © 2002 Elsevier Science B.V. All rights reserved.
DOI
10.1016/S0006-8993(02)03248-1
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
Files in This Item:
There are no files associated with this item.
Export
RIS (EndNote)
XLS (Excel)
XML


qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

BROWSE