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Gene expression profile of megakaryocytes from human cord blood CD34+ cells ex vivo expanded by thrombopoietin

Title
Gene expression profile of megakaryocytes from human cord blood CD34+ cells ex vivo expanded by thrombopoietin
Authors
Kim J.-A.Jung Y.-J.Seoh J.-Y.Woo S.-Y.Seo J.-S.Kim H.-L.
Ewha Authors
서주영김형래우소연
SCOPUS Author ID
서주영scopus; 김형래scopus; 우소연scopus
Issue Date
2002
Journal Title
Stem Cells
ISSN
1066-5099JCR Link
Citation
Stem Cells vol. 20, no. 5, pp. 402 - 416
Indexed
SCI; SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
Previously, we investigated the process of megakaryocytopoiesis during ex vivo expansion of human cord blood (CB) CD34+ cells using thrombopoietin (TPO) and found that megakaryocytopoiesis was closely associated with apoptosis. To understand megakaryocytopoiesis at the molecular level, we performed a microserial analysis of gene expression (microSAGE) in megakaryocytes (MKs) and nonmegakaryocytes (non-MKs) derived from human CB CD34+ cells by ex vivo expansion using TPO, and a total of 38,909 tags, representing 8,976 unique genes, were identified. In MKs, many of the known genes, including coagulation factor VII, P-selectin (CD62P),pim-1, azurocidin, defensin, and CD48 were highly expressed; meanwhile, those genes encoding some small G proteins of the Ras family (Rab 7 and Rab 11A) and glutathione S transferase family (1, 4, A2, omega, and pi) showed lower expression levels in MKs. These gene expression profiles will be useful to understand megakaryocytopoiesis at the molecular level, including apoptosis and related signal transduction pathways.
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의과대학 > 의학과 > Journal papers
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