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Changes of the level of G protein α-subunit mRNA by tolerance to and withdrawal from pentobarbital in rats

Title
Changes of the level of G protein α-subunit mRNA by tolerance to and withdrawal from pentobarbital in rats
Authors
Kim Y.Oh S.
Ewha Authors
오세관
SCOPUS Author ID
오세관scopus
Issue Date
2002
Journal Title
Neurochemical Research
ISSN
0364-3190JCR Link
Citation
vol. 27, no. 6, pp. 527 - 533
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Pentobarbital was continuously infused intracerebroventricularly (i.c.v.) at the rate of 300 μg/10 μl/h for 7 days, and withdrawal from pentobarbital was rendered 24 h after the stopping of the infusion. To eliminate the induction of hepatic metabolism by systemic administration of pentobarbital, an i.c.v. infusion model of tolerance to and withdrawal from pentobarbital was used. Little is known about the functional modulation of the G protein α-subunits at the molecular level. The effects of continuous infusion of pentobarbital on the modulation of G protein α-subunits mRNA were investigated by using in situ hybridization study. In situ hybridization showed that the level of Gαs mRNA was increased in the septum and brainstem, and the level of Gαo mRNA was elevated in the cortex during the pentobarbital withdrawal. The level of Gαi mRNA was significantly elevated in almost all area of brain during the pentobarbital withdrawal. These results suggest that region-specific changes of G protein α-subunit mRNA were involved in the withdrawal from pentobarbital, whereas α-subunit is not so highly involved in the pentobarbital tolerance.
DOI
10.1023/A:1019808905500
Appears in Collections:
의학전문대학원 > 의학과 > Journal papers
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