Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 서석효 | - |
dc.date.accessioned | 2016-08-28T11:08:03Z | - |
dc.date.available | 2016-08-28T11:08:03Z | - |
dc.date.issued | 2002 | - |
dc.identifier.issn | 0031-6768 | - |
dc.identifier.other | OAK-1136 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/219035 | - |
dc.description.abstract | We have studied the properties of a non-selective cation current (NSCCa) in macrovascular endothelial cells derived from human umbilical vein (EA cells) that is activated by an increase of intracellular Ca2+ concentration, [Ca2+]i. Current-voltage relationships are linear and the kinetics of the current is time-independent. Current-[Ca2+]i relationships were fitted to a Ca2+ binding site model with a concentration for half-maximal activation of 417±76 nM, a Hill coefficient of 2.3±0.8 and a maximum current of -23.9±2.7 pA/pF at -50 mV. The Ca2+-activated channel is more permeable to Na+ than for Cs+ (PCs/PNa=0.58, n=7), but virtually impermeable to Ca2+. Current activation was transient if ATP was omitted from the pipette solution. The maximal currents at 300 and 500 nM [Ca2+]i were smaller than in the absence of ATP, but were not significantly different at 2 μM. The intracellular Ca2+ concentration for half-maximal activation of the Ca2+-activated current was shifted to 811±12 nM in the absence of ATP. Substitution of ATP by the non-hydrolysable ATP analogue adenylylim-idodiphosphate (AMP-PNP) did not affect current activation. Sodium nitroprusside (SNP) decreased NSCCa in a concentration-dependent manner. The nitric oxide (NO) donors S-nitroso-N-acetylpenicillamine (SNAP) and 3-morpholinosydnonimine (SIN-1) also inhibited NSCCa. In contrast, nitro-L-arginine (NLA), which inhibits all NO-synthases, potentiated NSCCa, whereas superoxide dismutase (SOD), which inhibits the breakdown of NO, inhibited NSCCa. It is concluded that the Ca2+-activated non-selective action channel in EA cells is modulated by the metabolic state of the cell and by NO. | - |
dc.language | English | - |
dc.title | ATP and nitric oxide modulate a Ca2+-activated non-selective cation current in macrovascular endothelial cells | - |
dc.type | Article | - |
dc.relation.issue | 3 | - |
dc.relation.volume | 444 | - |
dc.relation.index | SCI | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 438 | - |
dc.relation.lastpage | 445 | - |
dc.relation.journaltitle | Pflugers Archiv European Journal of Physiology | - |
dc.identifier.doi | 10.1007/s00424-002-0825-x | - |
dc.identifier.wosid | WOS:000177153100017 | - |
dc.identifier.scopusid | 2-s2.0-0036451964 | - |
dc.author.google | Suh S.H. | - |
dc.author.google | Watanabe H. | - |
dc.author.google | Droogmans G. | - |
dc.author.google | Nilius B. | - |
dc.contributor.scopusid | 서석효(55666113100) | - |
dc.date.modifydate | 20230901081001 | - |