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Inhibitory effect of nikkomycin Z on chitin synthases in Candida albicans
- Inhibitory effect of nikkomycin Z on chitin synthases in Candida albicans
- Kim M.-K.; Park H.-S.; Kim C.-H.; Park H.-M.; Choi W.
- Ewha Authors
- SCOPUS Author ID
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- Journal Title
- vol. 19, no. 4, pp. 341 - 349
- SCI; SCIE; SCOPUS
- Nikkomycin Z is a competitive inhibitor of chitin synthases in fungi. It has been reported that it inhibits chitin synthases (Chs) 1 and 3, but not 2, of Saccharomyces cerevisiae. In our study, we found that: (a) nikkomycin Z inhibited all three Chs isozymes of Candida albicans (CaChs). The IC 50 value for CaChs1 is 15 μM, for CaChs2 0.8 μM, and for CaChs3 13 μM; (b) nikkomycin Z inhibits vegetative growth of C. albicans differently in different growth media; growth inhibition was observed on Spider and corn meal agar plate, but not on Lee's plate; (c) growth inhibition by nikkomycin Z accompanied by the absence of septum and cell wall chitin, which in turn brought about cell lysis. Nikkomycin Z did not lyse cells in Lee's media and lysis was partially prevented in the presence of sorbitol as an osmostabilizer in Spider medium. Therefore, nikkomycin Z prevented the formation of septum and cell wall chitin by inhibiting chitin synthase activities in a growth medium-dependent manner. Copyright © 2002 John Wiley & Sons, Ltd.
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