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Decrease in apoptosis and increase in polyploidization of megakaryocytes by stem cell factor during ex vivo expansion of human cord blood CD34+ cells using thrombopoietin
- Title
- Decrease in apoptosis and increase in polyploidization of megakaryocytes by stem cell factor during ex vivo expansion of human cord blood CD34+ cells using thrombopoietin
- Authors
- Kie J.-H.; Yang W.-I.; Lee M.-K.; Kwon T.-J.; Min Y.-H.; Kim H.-O.; Ahn H.-S.; Im S.-A.; Kim H.-L.; Park H.-Y.; Ryu K.-H.; Chung W.-S.; Shin M.-H.; Jung Y.-J.; Woo S.-Y.; Park H.-K.; Seoh J.-Y.
- Ewha Authors
- 박혜경; 박혜영; 서주영; 정화순; 유경하; 우소연
- SCOPUS Author ID
- 박혜경; 박혜영; 서주영; 정화순; 유경하; 우소연
- Issue Date
- 2002
- Journal Title
- Stem Cells
- ISSN
- 1066-5099
- Citation
- Stem Cells vol. 20, no. 1, pp. 73 - 79
- Indexed
- SCI; SCIE; SCOPUS
- Document Type
- Article
- Abstract
- Thrombopoietin (TPO) is widely used for ex vivo expansion of hematopoietic stem cells. Previously, we have reported that TPO induces a characteristic pattern of apoptosis, and the TPO-induced apoptosis is closely associated with megakaryocyte (MK) differentiation. In the present study, several cytokines, flt3-ligand, stem cell factor (SCF), interleukin-3 (IL-3), IL-6, IL-11, leukemia inhibitory factor, G-CSF, and erythropoietin, which are known to affect megakaryocytopoiesis, have been evaluated to elucidate their effects on the TPO-induced apoptosis. Measurement of apoptosis by flow cytometry revealed that only SCF absolutely reduced the TPO-induced apoptosis in MK fractions, particularly in the late phase of ex vivo expansion. Platelet production was demonstrated by electron microscopy in a later phase when SCF was added. Simultaneous measurement of DNA contents with immunophenotyping demonstrated a significant increase in polyploidization in the CD41+ cell fraction when cultured with SCF. These results suggested that SCF not only inhibited premature senescence but also enhanced maturation of the differentiating cells of MK lineage during ex vivo expansion using TPO.
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- 의과대학 > 의학과 > Journal papers
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