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Decrease in apoptosis and increase in polyploidization of megakaryocytes by stem cell factor during ex vivo expansion of human cord blood CD34+ cells using thrombopoietin

Title
Decrease in apoptosis and increase in polyploidization of megakaryocytes by stem cell factor during ex vivo expansion of human cord blood CD34+ cells using thrombopoietin
Authors
Kie J.-H.Yang W.-I.Lee M.-K.Kwon T.-J.Min Y.-H.Kim H.-O.Ahn H.-S.Im S.-A.Kim H.-L.Park H.-Y.Ryu K.-H.Chung W.-S.Shin M.-H.Jung Y.-J.Woo S.-Y.Park H.-K.Seoh J.-Y.
Ewha Authors
박혜경박혜영서주영정화순유경하우소연
SCOPUS Author ID
박혜경scopus; 박혜영scopus; 서주영scopus; 정화순scopus; 유경하scopus; 우소연scopus
Issue Date
2002
Journal Title
Stem Cells
ISSN
1066-5099JCR Link
Citation
vol. 20, no. 1, pp. 73 - 79
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Thrombopoietin (TPO) is widely used for ex vivo expansion of hematopoietic stem cells. Previously, we have reported that TPO induces a characteristic pattern of apoptosis, and the TPO-induced apoptosis is closely associated with megakaryocyte (MK) differentiation. In the present study, several cytokines, flt3-ligand, stem cell factor (SCF), interleukin-3 (IL-3), IL-6, IL-11, leukemia inhibitory factor, G-CSF, and erythropoietin, which are known to affect megakaryocytopoiesis, have been evaluated to elucidate their effects on the TPO-induced apoptosis. Measurement of apoptosis by flow cytometry revealed that only SCF absolutely reduced the TPO-induced apoptosis in MK fractions, particularly in the late phase of ex vivo expansion. Platelet production was demonstrated by electron microscopy in a later phase when SCF was added. Simultaneous measurement of DNA contents with immunophenotyping demonstrated a significant increase in polyploidization in the CD41+ cell fraction when cultured with SCF. These results suggested that SCF not only inhibited premature senescence but also enhanced maturation of the differentiating cells of MK lineage during ex vivo expansion using TPO.
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의과대학 > 의학과 > Journal papers
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