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약학대학
약학과
Journal papers
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Identification of structural domains affecting transactivation potential of Nm23
Title
Identification of structural domains affecting transactivation potential of Nm23
Authors
Cho S.-J.
;
Lee N.-S.
;
Jung Y.-S.
;
Lee H.
;
Lee K.-J.
;
Kim E.
;
Chae S.-K.
Ewha Authors
이공주
SCOPUS Author ID
이공주
Issue Date
2001
Journal Title
Biochemical and Biophysical Research Communications
ISSN
0006-291X
Citation
Biochemical and Biophysical Research Communications vol. 289, no. 3, pp. 738 - 743
Indexed
SCI; SCIE; SCOPUS
Document Type
Article
Abstract
The strong transactivation activity of the C-terminal half (amino acids 76-152) of Nm23 was reported previously. Here we examined a structural domain preventing or necessary to its transactivation activity. The C-terminal 1/4 (amino acids 109-152) was sufficient for transactivation, but the C-terminal half with a longer N-terminal extension (amino acids 58-152) caused the loss of the transactivation ability. Furthermore, coexpression of the N-terminal half with the C-terminus of Nm23-H1 blocked the transactivation activity of the C-terminal half, where direct interaction of both truncated proteins was demonstrated in vitro. Transactivation activities in the C-terminal halves of the known mutants (P96S, H118F, S120G, and S120A) exhibiting differential antimetastasis effects were also tested. Significant reduction of transactivation activity was observed only in H118F, indicating that NPD kinase active-site histidine is required. This suggests that transactivation potential of Nm23 is related to NDP kinase activity but not to metastasis suppressor activity. © 2001 Elsevier Science.
DOI
10.1006/bbrc.2001.6042
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