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dc.contributor.author오억수-
dc.date.accessioned2016-08-28T11:08:43Z-
dc.date.available2016-08-28T11:08:43Z-
dc.date.issued2001-
dc.identifier.issn0264-6021-
dc.identifier.otherOAK-735-
dc.identifier.urihttps://dspace.ewha.ac.kr/handle/2015.oak/218817-
dc.description.abstractFibronectin (FN) is known to transduce signal(s) to rescue cells from detachment-induced apoptosis (anoikis) through an integrin-mediated survival pathway. However, the functions of individual FN domains have not been studied in detail. In the present study we investigated whether the interaction of the cell-binding domain of FN with integrin is sufficient to rescue rat embryo fibroblasts (REFs) from detachment-induced apoptosis. REFs attached and spread normally after plating on substrates coated with either intact FN or a FN fragment, FN120, that contains the cell-binding domain but lacks the C-terminal heparin-binding domain, HepII. REFs on FN maintained a well-spread fibroblastic shape and even proliferated in serum-free medium at 20 h after plating. In contrast, previously well-spread REFs on FN120 started losing fibroblastic shape with time and detached from FN120-coated plates after approx. 8 h. Nuclear condensation indicated apototic cell death. This was due to the decreased activity/stability of focal adhesion kinase (pp125FAK) in the absence of HepII domain. A peptide in the HepII domain [peptide V, WQPPRARI (single-letter amino acid codes)], which has previously been implicated in cytoskeletal organization, rescued apoptotic changes. Consistently, pp125FAK phosphorylation was increased, and both cleavage of pp125FAK and activation of caspase 3 on FN120 were partly blocked by peptide V. Thus the interaction of the cell-binding domain with integrin has a major role in cell survival but is itself not sufficient for cell survival. One or more additional survival signals come from the HepII domain to regulate pp125FAK activity/stability.-
dc.languageEnglish-
dc.titleRat embryo fibroblasts require both the cell-binding and the heparin-binding domains of fibronectin for survival-
dc.typeArticle-
dc.relation.issue2-
dc.relation.volume356-
dc.relation.indexSCIE-
dc.relation.indexSCOPUS-
dc.relation.startpage531-
dc.relation.lastpage537-
dc.relation.journaltitleBiochemical Journal-
dc.identifier.doi10.1042/0264-6021:3560531-
dc.identifier.wosidWOS:000169295800028-
dc.identifier.scopusid2-s2.0-0035366676-
dc.author.googleJeong J.-
dc.author.googleHan I.-
dc.author.googleLim Y.-
dc.author.googleKim J.-
dc.author.googlePark I.-
dc.author.googleWoods A.-
dc.author.googleCouchman J.R.-
dc.author.googleOh E.-S.-
dc.contributor.scopusid오억수(7101967153)-
dc.date.modifydate20190901081003-
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자연과학대학 > 생명과학전공 > Journal papers
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