Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 오억수 | - |
dc.date.accessioned | 2016-08-28T11:08:43Z | - |
dc.date.available | 2016-08-28T11:08:43Z | - |
dc.date.issued | 2001 | - |
dc.identifier.issn | 0264-6021 | - |
dc.identifier.other | OAK-735 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/218817 | - |
dc.description.abstract | Fibronectin (FN) is known to transduce signal(s) to rescue cells from detachment-induced apoptosis (anoikis) through an integrin-mediated survival pathway. However, the functions of individual FN domains have not been studied in detail. In the present study we investigated whether the interaction of the cell-binding domain of FN with integrin is sufficient to rescue rat embryo fibroblasts (REFs) from detachment-induced apoptosis. REFs attached and spread normally after plating on substrates coated with either intact FN or a FN fragment, FN120, that contains the cell-binding domain but lacks the C-terminal heparin-binding domain, HepII. REFs on FN maintained a well-spread fibroblastic shape and even proliferated in serum-free medium at 20 h after plating. In contrast, previously well-spread REFs on FN120 started losing fibroblastic shape with time and detached from FN120-coated plates after approx. 8 h. Nuclear condensation indicated apototic cell death. This was due to the decreased activity/stability of focal adhesion kinase (pp125FAK) in the absence of HepII domain. A peptide in the HepII domain [peptide V, WQPPRARI (single-letter amino acid codes)], which has previously been implicated in cytoskeletal organization, rescued apoptotic changes. Consistently, pp125FAK phosphorylation was increased, and both cleavage of pp125FAK and activation of caspase 3 on FN120 were partly blocked by peptide V. Thus the interaction of the cell-binding domain with integrin has a major role in cell survival but is itself not sufficient for cell survival. One or more additional survival signals come from the HepII domain to regulate pp125FAK activity/stability. | - |
dc.language | English | - |
dc.title | Rat embryo fibroblasts require both the cell-binding and the heparin-binding domains of fibronectin for survival | - |
dc.type | Article | - |
dc.relation.issue | 2 | - |
dc.relation.volume | 356 | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 531 | - |
dc.relation.lastpage | 537 | - |
dc.relation.journaltitle | Biochemical Journal | - |
dc.identifier.doi | 10.1042/0264-6021:3560531 | - |
dc.identifier.wosid | WOS:000169295800028 | - |
dc.identifier.scopusid | 2-s2.0-0035366676 | - |
dc.author.google | Jeong J. | - |
dc.author.google | Han I. | - |
dc.author.google | Lim Y. | - |
dc.author.google | Kim J. | - |
dc.author.google | Park I. | - |
dc.author.google | Woods A. | - |
dc.author.google | Couchman J.R. | - |
dc.author.google | Oh E.-S. | - |
dc.contributor.scopusid | 오억수(7101967153) | - |
dc.date.modifydate | 20190901081003 | - |