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Inhibition of breast cancer growth in vivo by antiangiogenesis gene therapy with adenovirus-mediated antisense-VEGF

Title
Inhibition of breast cancer growth in vivo by antiangiogenesis gene therapy with adenovirus-mediated antisense-VEGF
Authors
Im S.-A.Kim J.-S.Gomez-Manzano C.Fueyo J.Liu T.-J.Cho M.-S.Seong C.-M.Lee S.N.Hong Y.-K.Yung W.K.A.
Ewha Authors
성주명조민선
SCOPUS Author ID
성주명scopus; 조민선scopus
Issue Date
2001
Journal Title
British Journal of Cancer
ISSN
0007-0920JCR Link
Citation
vol. 84, no. 9, pp. 1252 - 1257
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Increased expression of VEGF in several types of tumours has been shown to correlate with poor prognosis. We used a replication-deficient adenoviral vector containing antisense VEGF cDNA (Ad5CMV-αVEGF) to down-regulate VEGF expression and increase the efficiency of delivery of the antisense sequence in the human breast cancer cell line MDA231-MB. Transfection of these cells with Ad5CMV-αVEGF in vitro reduced secreted levels of VEGF protein without affecting cell growth. Moreover, injection of the Ad5CMV-αVEGF vector into intramammary xenografts of these cells established in nude mice inhibited tumour growth and reduced the amount of VEGF protein and the density of microvessels in those tumours relative to tumours treated with the control vector Ad5(dl312). Our results showed that antisense VEGF 165 cDNA was efficiently delivered in vivo via an adenoviral vector and that this treatment significantly inhibited the growth of established experimental breast tumours. The Ad5CMV-αVEGF vector may be useful in targeting the tumour vasculature in the treatment of breast cancer. © 2001 Cancer Research Campaign.
DOI
10.1054/bjoc.2000.1734
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의학전문대학원 > 의학과 > Journal papers
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