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Capsaicin inhibits the voltage-operated calcium channels intracellularly in the antral circular myocytes of guinea-pig stomach

Title
Capsaicin inhibits the voltage-operated calcium channels intracellularly in the antral circular myocytes of guinea-pig stomach
Authors
Sim J.H.Kim C.K.Kim S.J.Lee S.J.Suh S.H.Jun J.Y.So I.Kim K.W.
Ewha Authors
서석효
SCOPUS Author ID
서석효scopus
Issue Date
2001
Journal Title
Life Sciences
ISSN
0024-3205JCR Link
Citation
vol. 68, no. 21, pp. 2347 - 2360
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Studies of the effect of capsaicin (CAP) on the smooth muscle contractions have shown both contraction and relaxation in various preparations. The direct effect of CAP on gastric smooth muscle itself has not yet been reported, though CAP was reported to relax the isolated guinea-pig stomach by releasing nitric oxide from the CAP-sensitive sensory neurons. Here we showed an evidence that CAP evokes a prolonged relaxation of gastric antral circular smooth muscle(CAP-induced relaxation) by blocking the voltage-operated Ca2+ channels (VOCC) from inside of the cell. CAP suppressed dose-dependently the spontaneous contractions of guinea-pig gastric circular muscle strip under the condition without neural influence (IC50 = 5.8 μM). The inhibitory effects of CAP both on the high K+ contracture induced by 50 mM K+ Tyrode solution and on the slow waves recorded using a conventional intracellular microelectrode technique were similar to those of Ca2+ channel antagonists, indicating that Ca2+ influx through the VOCC is decreased by CAP. Ca2+ channel current (IBa) decreased in a concentration-dependent manner on superfusing the physiological salt solution containing various concentrations of CAP. The steady-state activation and inactivation curves of IBa were not affected by the treatment with CAP. The experiment using a synthetic water-soluble analog of CAP, DA-5018·HCl, suggested that the acting site of CAP is present in the intracellular side. Spontaneous transient outward K+ currents(STOCs) recorded at a holding potential of 0 mV were also inhibited by CAP and verapamil, Ca channel blocker. Taken together, these results indicate that CAP-induced relaxation is associated with the direct inhibitory action on the VOCC from inside of the cell. © 2001 Elsevier Science Inc.
DOI
10.1016/S0024-3205(01)01027-X
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의학전문대학원 > 의학과 > Journal papers
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