Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 안정자 | * |
dc.contributor.author | 정혜원 | * |
dc.contributor.author | 문혜성 | * |
dc.date.accessioned | 2016-08-28T11:08:38Z | - |
dc.date.available | 2016-08-28T11:08:38Z | - |
dc.date.issued | 2001 | * |
dc.identifier.issn | 0021-972X | * |
dc.identifier.other | OAK-648 | * |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/218765 | - |
dc.description.abstract | The interleukin-1 (IL-1) system plays an integral role in local intercellular interactions during implantation. In addition, the plasminogen activator system, especially urokinase plasminogen activator (u-PA), plasminogen activator inhibitor (PAI-1), and u-PA receptor (u-PAR), are crucial during embryo implantation. Decidualization and implantation are complex processes dependent upon several proteases, including u-PA, and IL-1 is known to affect PA activity in several cell types. We investigated the role of IL-1β in regulating u-PA, PAI-1, u-PAR, and soluble u-PAR messenger ribonucleic acid (mRNA) expression in cultured human endometrial stromal cells using quantitative competitive PCR. For confirmation of the mRNA data, we measured PAI-1 and u-PAR protein by enzyme-linked immunosorbent assay. Confluent stromal cell cultures treated with progesterone and estradiol for 9 days were stimulated with IL-1β, and IL-1β plus IL-1β antibody for an additional 24 h. Total RNA was extracted, reverse transcribed, and coamplified using quantitative and competitive PCR with internal standards. IL-1β increased PAI-1, u-PAR, and soluble u-PAR expression in a dose-dependent manner, and this result was reversed by anti-IL-1β antibody treatment, u-PA mRNA expression was not dependent on IL-1β. These results suggest that IL-1 may be important in regulating PAI-1 and u-PAR during stromal cell decidualization before implantation. | * |
dc.language | English | * |
dc.title | Interleukin-1β regulates urokinase plasminogen activator (u-PA), u-PA receptor, soluble u-PA receptor, and plasminogen activator inhibitor-1 messenger ribonucleic acid expression in cultured human endometrial stromal cells | * |
dc.type | Article | * |
dc.relation.issue | 3 | * |
dc.relation.volume | 86 | * |
dc.relation.index | SCI | * |
dc.relation.index | SCIE | * |
dc.relation.index | SCOPUS | * |
dc.relation.startpage | 1332 | * |
dc.relation.lastpage | 1340 | * |
dc.relation.journaltitle | Journal of Clinical Endocrinology and Metabolism | * |
dc.identifier.doi | 10.1210/jc.86.3.1332 | * |
dc.identifier.wosid | WOS:000167512000061 | * |
dc.identifier.scopusid | 2-s2.0-0034744569 | * |
dc.author.google | Chung H.-W. | * |
dc.author.google | Wen Y. | * |
dc.author.google | Ahn J.-J. | * |
dc.author.google | Moon H.-S. | * |
dc.author.google | Polan M.L. | * |
dc.contributor.scopusid | 안정자(7403019711) | * |
dc.contributor.scopusid | 정혜원(56499737200) | * |
dc.contributor.scopusid | 문혜성(14319143700) | * |
dc.date.modifydate | 20240415131357 | * |