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Identification of the self-interaction of rat TCTP/IgE-dependent histamine-releasing factor using yeast two-hybrid system

Title
Identification of the self-interaction of rat TCTP/IgE-dependent histamine-releasing factor using yeast two-hybrid system
Authors
Yoon T.Jung J.Kim M.Kang Man LeeEung Chil ChoiLee K.
Ewha Authors
이강만이경림윤태숙
SCOPUS Author ID
이강만scopus; 이경림scopus
Issue Date
2000
Journal Title
Archives of Biochemistry and Biophysics
ISSN
0003-9861JCR Link
Citation
vol. 384, no. 2, pp. 379 - 382
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
To further understand the biological function of translationally controlled tumor protein (TCTP), also known as IgE-dependent histamine-releasing factor (HRF), the yeast two-hybrid system was used to screen interacting molecules. We isolated cDNA clones coding for TCTP/HRF, suggesting that it may have a self-interacting property. Domain mapping of the interaction revealed that the C-terminal region of residue 126-172 is involved in self-interaction. The self-interacting property of TCTP/HRF was further supported by FPLC gel-filtration chromatography and coimmunoprecipitation analysis from transfected COS-7 cells. Our data suggests that TCTP/HRF may have a potential to self-interact through the C-terminal region, and the self-interaction property may be related to its biological function. © 2000 Academic Press.
DOI
10.1006/abbi.2000.2108
Appears in Collections:
약학대학 > 약학과 > Journal papers
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