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Identification of the Calcium Binding Sites in Translationally Controlled Tumor Protein

Title
Identification of the Calcium Binding Sites in Translationally Controlled Tumor Protein
Authors
Kim M.Jung Y.Lee K.Kim C.
Ewha Authors
김춘미이경림
SCOPUS Author ID
김춘미scopus; 이경림scopus
Issue Date
2000
Journal Title
Archives of Pharmacal Research
ISSN
0253-6269JCR Link
Citation
vol. 23, no. 6, pp. 633 - 636
Indexed
SCIE; SCOPUS; KCI WOS scopus
Abstract
Translationally controlled tumor protein (TCTP), also known as IgE-dependent histamine-releasing factor, is a growth-related tumor protein. Although the primary sequence of rat TCTP does not reveal any recognizable Ca2+ -binding motif, previous studies have demonstrated that rat TCTP consisting of 172 amino acids is a Ca2+ -binding protein. However, the region of TCTP required for Ca2+ interaction has not been mapped to the molecule. Here, we reported that the Ca2+ binding region of TCTP, which was mapped by using a combination of deletion constructs of rat TCTP and 45Ca2+-overlay assay, was confined to amino acid residues 81-112. This binding domain did not show any peculiar loop of calcium-binding motif such as CaLB domain and EF hand motif and it seems to be constituted of random coil regions neighboring the a helix. Thus, our data confirm that TCTP is a novel family of Ca2+-binding protein.
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약학대학 > 약학과 > Journal papers
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