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Transforming growth factor-β induces apoptosis in activated murine T cells through the activation of caspase 1-1ike protease

Title
Transforming growth factor-β induces apoptosis in activated murine T cells through the activation of caspase 1-1ike protease
Authors
Chung E.J.Choi S.H.Shim Y.-H.Bang Y.-J.Hur K.C.Kim C.W.
Ewha Authors
허규정
SCOPUS Author ID
허규정scopus
Issue Date
2000
Journal Title
Cellular Immunology
ISSN
0008-8749JCR Link
Citation
vol. 204, no. 1, pp. 46 - 54
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
Transforming growth factor-β (TGF-β) has been known as a potent immunosuppressive cytokine that can induce apoptosis in lymphoid cells. We established an IL-2-independent cell line, CTLL-2A, from murine T cell line CTLL-2. CTLL-2A expressed higher levels of CD95, CD69, and CD18 molecules than CTLL-2 did, suggesting a more activated state in CTLL-2A than in the CTLL-2 by phenotype. Exposing both CTLL-2 and CTLL-2A to TGF-β results in differential apoptosis patterns defined by DNA fragmentation and plasma membrane alteration. Among the bcl-2 family members, bcl-2, bcl-w, and bcl-x(L) were also differently expressed in these two cell lines. In CTLL-2A, bcl-x(L) was amplified as a major anti-apoptotic molecule, and TGF-β-induced cell death was more enhanced than in the original cell line. Caspase 1-like protease was activated by TGF-β treatment and consequently it cleaved bcl-x(L) in CTLL-2A. TGF-β-induced DNA fragmentation and cleavage of bcl-x(L) were inhibited by pretreatment with tetra peptide caspase 1 inhibitor, YVAD.cmk. These findings suggest that TGF-β induces cell death in activated murine T cells through cleavage of bcl-x(L) via activated caspase 1-like protease, which may act as an important executor in that process. (C) 2000 Academic Press.
DOI
10.1006/cimm.2000.1694
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일반대학원 > 바이오융합과학과 > Journal papers
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