Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 신윤용 | - |
dc.date.accessioned | 2016-08-28T11:08:25Z | - |
dc.date.available | 2016-08-28T11:08:25Z | - |
dc.date.issued | 2000 | - |
dc.identifier.issn | 0918-6158 | - |
dc.identifier.other | OAK-424 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/218633 | - |
dc.description.abstract | Since it is known that hypoxia increases inducible nitric oxide synthase (iNOS) gene expression through the hypoxia responsive element, it was hypothesized that nitric oxide could be a mediator of hypoxia to inhibit Cyp1a1 promoter activity. In order to test this hypothesis, we have undertaken a study to examine the effects of hypoxia and nitric oxide on Cyp1a1 promoter activity in Hepa I cells. Mouse Cyp1a1 5' flanking DNA, 1.6 kb, was cloned into pGL3 expression vector in order to construct pmCyp1a1- Luc. Hepa I cells were transfected with pmCyp1a1-Luc and were treated with 10-9M 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in the presence or absence of various hypoxic agents such as 10-6-10-4M cobalt chloride or 10-6- 10-4M picolinic acid or 10-6-10-4 M desferrioxamine. The luciferase activity of the reporter gene was measured from pm Cyp1a1-Luc transfected Hepa I cell lysate which contains 2 μg total protein using luciferin as a substrate. Hypoxic agents such as cobalt chloride, picolinic acid, and desferrioxamine showed inhibition of luciferase activity that was induced by 10-9 M TCDD treatment in a dose dependent manner. Concomitant treatment of 1 mM N(G)-nitro-l-arginine with 10-6-10-4M cobalt chloride or 10-6-10-4M desferrioxamine or 10-6-10-4M picolinic acid or 10-6-10-4M sodium nitroprusside recovered luciferase activity from the TCDD induced luciferase activity that was inhibited by hypoxic agents. These data demonstrated that nitric oxide might be a mediator of iron chelating agents and hypoxic agents to inhibit dioxin induced Cyp1a1 promoter activity in Hepa I cells. | - |
dc.language | English | - |
dc.title | Nitric oxide inhibits dioxin action for the stimulation of Cyp1a1 promoter activity | - |
dc.type | Article | - |
dc.relation.issue | 5 | - |
dc.relation.volume | 23 | - |
dc.relation.index | SCI | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 575 | - |
dc.relation.lastpage | 580 | - |
dc.relation.journaltitle | Biological and Pharmaceutical Bulletin | - |
dc.identifier.wosid | WOS:000086890100013 | - |
dc.identifier.scopusid | 2-s2.0-0034113656 | - |
dc.author.google | Kim J.-E. | - |
dc.author.google | Sheen Y.Y. | - |
dc.contributor.scopusid | 신윤용(6603872711) | - |
dc.date.modifydate | 20230411104830 | - |