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Platelet-derived growth factor-induced H2O2 production requires the activation of phosphatidylinositol 3-kinase

Title
Platelet-derived growth factor-induced H2O2 production requires the activation of phosphatidylinositol 3-kinase
Authors
Bae Y.S.Sung J.-Y.Kim O.-S.Kim Y.J.Hur K.C.Kazlauskas A.Rhee S.G.
Ewha Authors
허규정배윤수
SCOPUS Author ID
허규정scopus; 배윤수scopus
Issue Date
2000
Journal Title
Journal of Biological Chemistry
ISSN
0021-9258JCR Link
Citation
Journal of Biological Chemistry vol. 275, no. 14, pp. 10527 - 10531
Indexed
SCIE; SCOPUS WOS scopus
Document Type
Article
Abstract
Autophosphorylation of the platelet-derived growth factor (PDGF) receptor triggers intracellular signaling cascades as a result of recruitment of Src homology 2 domain-containing enzymes, including phosphatidyl-inositol 3-kinase (PI3K), the GTPase-activating protein of Ras (GAP), the protein- tyrosine phosphatase SHP-2, and phospholipase C-γ1 (PLC-γ1), to specific phosphotyrosine residues. The roles of these various effectors in PDGF- induced generation of H2O2 have now been investigated in HepG2 cells expressing various PDGF receptor mutants. These mutants included a kinase- deficient receptor and receptors in which various combinations of the tyrosine residues required for the binding of PI3K (Tyr740 and Tyr751), GAP (Tyr771), SHP-2 (Tyr1009), or PLC-γ1 (Tyr1021) were mutated to Phe. PDGF failed to increase H2O2 production in cells expressing either the kinase-deficient mutant or a receptor in which the two Tyr residues required for the binding of PI3K were re- placed by Phe. In contrast, PDGF- induced H2O2 production in cells expressing a receptor in which the binding sites for GAP, SHP-2, and PLC-γ1 were all mutated was slightly greater than that in cells expressing the wild-type receptor. Only the PI3K binding site was alone sufficient for PDGF-induced H2O2 production. The effect of PDGF on H2O2 generation was blocked by the PI3K inhibitors LY294002 and wortmannin or by overexpression of a dominant negative mutant of Rac1. These results suggest that a product of PI3K is required for PDGF-induced production of H2O2 in nonphagocytic cells, and that Rac1 mediates signaling between the PI3K product and the putative NADPH oxidase.
DOI
10.1074/jbc.275.14.10527
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일반대학원 > 바이오융합과학과 > Journal papers
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