Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 정영해 | - |
dc.date.accessioned | 2016-08-28T11:08:24Z | - |
dc.date.available | 2016-08-28T11:08:24Z | - |
dc.date.issued | 2000 | - |
dc.identifier.issn | 1355-0284 | - |
dc.identifier.other | OAK-403 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/218624 | - |
dc.description.abstract | In attempts to elucidate the pathogenic mechanisms involved in neurodegeneration in AIDS patients with cognitive deficits, the possible effect of HIV-1 transmembrane envelope protein gp41 on expression of the membrane inhibitor of complement mediated cytolysis (CD59) was assessed in human neuronal (SK-N-SH) and astroglial (T98G) cell lines. Western blotting analyses demonstrated that an immunodominant (ID, aa 598-613) gp41 peptide as well as the recombinant gp41 protein encompassing this domain markedly reduced CD59 level in a dose dependent manner whereas p24 and control peptide had little effect. RT-PCR showed that ID peptide also elicited a reduction in the expressed CD59 mRNA level. This gp41 peptide apparently down-regulated phorbol 12,13-dibutyrate induced elevation of CD59 at the protein and mRNA levels in a manner similar to that conferred by protein kinase C inhibitor, H-7 or staurosporine in SK-N-SH. Interestingly, proinflammatory cytokines such as IL-1β or IFN-γ as well as LPS greatly decreased CD59 in SK-N-SH and to a lesser extent in T98G whereas TNF-α did not significantly alter it. In contrast, antioxidants and anti-inflammatory agents enhanced CD59 expression reversing gp41 peptide mediated inhibitory effect in SK-N-SH. Our data suggest that high level of gp41 or its metabolites as well as impaired protein kinase response, chronic inflammation or antioxidant depletion within HIV-1 infected brains may be associated with a diminished expression of CD59 which would render neuronal cells to susceptible to indirect bystander lysis in the presence of autologous complement. | - |
dc.language | English | - |
dc.title | Expression of complement inhibitor protein CD59 in human neuronal and glial cell lines treated with HIV-1 gp41 peptides | - |
dc.type | Article | - |
dc.relation.issue | 1 | - |
dc.relation.volume | 6 | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 51 | - |
dc.relation.lastpage | 60 | - |
dc.relation.journaltitle | Journal of NeuroVirology | - |
dc.identifier.wosid | WOS:000086344800007 | - |
dc.identifier.scopusid | 2-s2.0-0034107257 | - |
dc.author.google | Chong Y.H. | - |
dc.author.google | Lee M.J. | - |
dc.contributor.scopusid | 정영해(7201371824) | - |
dc.date.modifydate | 20180901081003 | - |