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Identification and characterization of human Fas associated factor 1, hFAF1

Title
Identification and characterization of human Fas associated factor 1, hFAF1
Authors
Ryu S.-W.Chae S.-K.Lee K.-J.Kim E.
Ewha Authors
이공주
SCOPUS Author ID
이공주scopus
Issue Date
1999
Journal Title
Biochemical and Biophysical Research Communications
ISSN
0006-291XJCR Link
Citation
vol. 262, no. 2, pp. 388 - 394
Indexed
SCI; SCIE; SCOPUS WOS scopus
Abstract
We have identified and characterized a cDNA encoding human Fas associated factor 1 (hFAF1) cDNA and a shorter form of hFAF1 cDNA [hFAF1(s)] with a 456 bp internal in-frame deletion from a human HeLa cDNA library. The nucleotide sequences of hFAF1 and hFAF1(s) were identical except for the deletion. GST-hFAF1 fusion protein bound to the in vitro translation product of Fas. The N-terminal region (amino acid 1~201) including the upstream ubiquitin homology domain of hFAF1 could bind with the death domain of Fas unlike that of qFAF1 whose binding region with Fas could not be determined. However hFAF1 did not bind to the death domain of Fas mutant, lpr(cg). hFAF1 was expressed abundantly in testis, skeletal muscle, and heart as 2.8 kb mRNA. Polyclonal antibody against hFAF1 detected 74 kD protein, a deduced protein size from the ORF and 40 kD protein in some cell lines.
DOI
10.1006/bbrc.1999.1217
Appears in Collections:
약학대학 > 약학과 > Journal papers
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