Full metadata record
DC Field | Value | Language |
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dc.contributor.author | 서석효 | - |
dc.date.accessioned | 2016-08-28T11:08:04Z | - |
dc.date.available | 2016-08-28T11:08:04Z | - |
dc.date.issued | 1998 | - |
dc.identifier.issn | 0031-6768 | - |
dc.identifier.other | OAK-32 | - |
dc.identifier.uri | https://dspace.ewha.ac.kr/handle/2015.oak/218411 | - |
dc.description.abstract | The possibility of the protein kinase C (PKC) pathway being a mechanism underlying the desensitization of carbachol- (CCh-)activated nonselective cationic current (I(CCh)) was investigated in a study of guinea-pig gastric myocytes. Using the conventional whole-cell patch-clamp technique with symmetrical CsCl-rich solution in pipette and bath, I(CCh) was induced by bath application of 50 μM CCh. With 0.5 mM EGTA [ethyleneglycol-bis(β-aminoethyl ether)-N,N,N',N'-tetraacetic acid] in the pipette solution (0.5 mM [EGTA](i)), I(CCh) decayed spontaneously (desensitization of I(CCh)) to around 20% within 10 min. Desensitization of I(CCh) was significantly attenuated with 2 mM [EGTA](i). At a concentration of 20 μM OAG (1-oleoyl-2-acetyl-sn-glycerol), a PKC activator, inhibited I(CCh) at 0.5 mM [EGTA](i) but far less at 2 mM [EGTA](i) (18% and 81% of control, respectively). The same cationic current induced by intracellular guanosine-5'-O-(3-thiotriphosphate) (GTP[γ-S]) was not inhibited by OAG with 0.5 mM [EGTA](i). The pretreatment of gastric myocytes with PKC inhibitors, either 1 μM chelerythrine or 1 μM peptide inhibitor, attenuated the desensitization of I(CCh). [Ca2+](i) was also measured by single cell microfluorometry using fura-2. Under CCh stimulation with 2 mM [EGTA](i), [Ca2+](i) did not increase above 100 nM while it increased to around 260 nM with 0.5 mM [EGTA](i). These results suggest that the desensitization of I(CCh) is partly due to the Ca2+-dependent PKC pathway in guinea-pig gastric myocytes. | - |
dc.language | English | - |
dc.title | Protein kinase C mediates the desensitization of CCh-activated nonselective cationic current in guinea-pig gastric myocytes | - |
dc.type | Article | - |
dc.relation.issue | 1 | - |
dc.relation.volume | 436 | - |
dc.relation.index | SCI | - |
dc.relation.index | SCIE | - |
dc.relation.index | SCOPUS | - |
dc.relation.startpage | 1 | - |
dc.relation.lastpage | 8 | - |
dc.relation.journaltitle | Pflugers Archiv European Journal of Physiology | - |
dc.identifier.doi | 10.1007/s004240050597 | - |
dc.identifier.wosid | WOS:000073618700001 | - |
dc.identifier.scopusid | 2-s2.0-0031976360 | - |
dc.author.google | Kim Y.C. | - |
dc.author.google | Kim S.J. | - |
dc.author.google | Sim J.H. | - |
dc.author.google | Jun J.Y. | - |
dc.author.google | Kang T.M. | - |
dc.author.google | Suh S.H. | - |
dc.author.google | So I. | - |
dc.author.google | Kim K.W. | - |
dc.contributor.scopusid | 서석효(55666113100) | - |
dc.date.modifydate | 20230901081001 | - |